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T6674 Sigma-Aldrich

Tumor Necrosis Factor-α human

TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym: TNF-α, hTNF-α

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Properties

Related Categories Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Core Bioreagents,
Quality Level   200
biological source   human
recombinant   expressed in E. coli
assay   ≥97% (SDS-PAGE)
form   powder
potency   0.02-0.3 ng/mL ED50/EC50
quality   endotoxin tested
mol wt   ~17.4 kDa
packaging   pkg of 5x10 μg
  pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
application(s)   cell culture | mammalian: suitable
impurities   <1 EU/μg
UniProt accession no.   P01375
storage temp.   −20°C
Gene Information   human ... TNF(7124)

Description

Application

TNF-α include stimulating growth of human fibroblasts and other cell lines activating polymorphonuclear neutrophils and osteoclasts and inducing interleukin-1, prostaglandin E2, and collagenase production.

Tumor Necrosis Factor-α (TNFα) human has been used:
• to analyze the effects of cytokine TNFα-stressed human neuronal and glial (HNG) cells
• to investigate the molecular mechanisms of TNFα-mediated prolyl-4 hydroxylase α1 (P4Hα1) suppression
• to induce death-receptor-mediated apoptosis in HeLa cells.

Biochem/physiol Actions

Tumore necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is N-glycosylated.

Physical form

Lyophilized from a 0.2 μm-filtered buffer solution containing 3mM Tris-HCl and 5% trehalose.

Analysis Note

The biological activity of TNF-α is measured by the cytolysis of murine L929 cells.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the package size of Product T6674, Tumor Necrosis Factor-α human?
It is a 10μg size-power form.
What are the units/mg of Product T6674, Tumor Necrosis Factor-α human?
1ug=1.1x10e5 units.
What is the solubility of this Product T6674, Tumor Necrosis Factor-α human?
This can be dissolved at 0.1 to 1.0 mg/ml in saline or buffer, stabilized with 0.1 to 1%BSA.
What is the EC50 of Product T6674, Tumor Necrosis Factor-α human?
Bioactivity(cell inhibition, L929 cells) is expressed by an EC50 of 0.02 to 0.05 ng/mL for T6674.EC50 (equivalent concentration) is the same as ED50 (equivalent dose, cytotoxicity, L929 cells) used for the bioactivity specification for T0157, Tumor Necrosis Factor-alpha, human recombinant expressed in Yeast.
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Peer-Reviewed Papers
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