T8787 Sigma-Aldrich

Triton X-100

for molecular biology

Synonym: t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

  • CAS Number 9002-93-1

  • Linear Formula t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10

  •  Beilstein/REAXYS Number 2315025

  •  MDL number MFCD00128254

  •  PubChem Substance ID 329826885

  •  NACRES NA.21



Related Categories Antigen-Vaccine Preparation, Apoptosis and Cell Cycle, Biochemicals, Biochemicals and Reagents, Cell Biology,
Quality Level   200
grade   for molecular biology
description   non-ionic
form   liquid
mol wt   micellar avg mol wt 80,000
  average mol wt 625
aggregation number   100-155
application(s)   hematology: suitable
  histology: suitable
  protein purification: suitable
  protein quantification: suitable
  western blot: suitable
pH   9.7
CMC   0.2-0.9 mM (20-25°C)
mp   6 °C
transition temp   cloud point 65 °C
  pour point ~7 °C
solubility   water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
density   1.06 g/mL at 25 °C (lit.)
HLB   13.5
Featured Industry   Diagnostic Assay Manufacturing
foreign activity   DNase and RNase, none detected
storage temp.   room temp
InChI   1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3


General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.


Triton X-100 has been used:
• In immunohistochemistry for staining the Flat-mount retinas
• Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
• To permeabilise cells during Immunofluorescent microscopic studies
• As a positive control in LDH assay to determine the cell membrane integrity
• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
• For the preparation of outer membrane protein exctract
• As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells
• In the treatment of tissue sections for Immunofluorescence labeling


50, 100, 250 mL in poly bottle

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
UN 3082 9 / PGIII
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

pHast Pack
Protocols & Articles


3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular materia...
Keywords: Adhesion, Cancer, Cell attachment, Cell culture, Cryopreservation, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Vitamins

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot


Protein Extraction and Cell Lysis Protocol

All the steps for protein extraction from cells or tissue (fresh or frozen) must be carried out at 2-8 °C. The following is the composition of one common lysis buffer that is used to prepare protein ...
Keywords: BCA assay, Cell disruption, Electrophoresis, Gel electrophoresis, Homogenization, Protein assay, Protein extraction

Peer-Reviewed Papers


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