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T9253 Sigma-Aldrich

Trypsin inhibitor from chicken egg white

Type II-O, Partially purified ovomucoid, containing ovoinhibitor

Synonym: Ovomucoid

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Properties

Related Categories A to C, Biochemicals and Reagents, Chymotrypsin, α-, Chymotrypsin, alpha-, Core Bioreagents,
Quality Level   200
biological source   chicken egg white
type   Type II-O
form   powder
mol wt   14 kDa
solubility   water: soluble 10 g/L
storage temp.   2-8°C

Description

General description

25% of ovomucoid comprises carbohydrate.

Ovomucoid or trypsin inhibitor is an abundant protein in most avian egg whites. The hen′s egg protein is composed of about 186 amino acid residues, and is highly glycosylated. Three tandem domains are each homologous with pancreatic trypsin inhibitor. It is highly immunogenic, and probably accounts for most cases of egg allergy.

Application

Trypsin inhibitor from chicken egg white has been used:
• to produce denatured, oxidized and deglycosylated ovomucoid and used to stimulate patient derived cell cultures for mapping T cell epitopes
• to treat nuclei for flow cytometric analysis
• in Holtfreter′s solution, used for the dissociation of planarians into cells

Packaging

1, 5 g in poly bottle

250, 500 mg in poly bottle

Unit Definition

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Analysis Note

One mg will inhibit 0.8-1.6 mg of tryspin with activity of approx. 10,000 BAEE units per mg protein. May inhibit ≤0.3 mg of chymotrypsin with activity of approx. 40 BTEE units per mg protein.

Other Notes

Ovomucoid, from chicken egg white, does not itself inhibit chymotrypsin. Chymotrypsin inhibition is a measure of ovoinhibitor contamination by method of Feeney, R. E., et al., J. Biol. Chem., 238, 1415 (1963).

View more information on Trypsin Inhibitor.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)
Keywords: Chromatography, High performance liquid chromatography, Separation

Protocols

Enzymatic Assay of Trypsin Inhibitor

3.2 Unit Definition – One Trypsin BAEE unit will produce a ΔA253nm of 0.001 per minute with BAEE as substrate at pH 7.6 at 25ºC in a reaction volume of 3.2 ml.

Peer-Reviewed Papers
15

References

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