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T9822 Sigma-Aldrich

Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse

clone B3, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
conjugate   unconjugated
clone   B3, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 0.5-1 μg/mL using cytosolic fraction of rat brain extract
species reactivity   rabbit, rat, chicken, mouse, bovine, human, Oxyrrhis marina (dinoflagellate), monkey, sea urchin (Lytechinus pictus and three others.)
mol wt   antigen ~50 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgM
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P68363
Gene Information   human ... TUBA4A(7277), TUBB(203068)
mouse ... Tuba1a(22142), Tubb2b(73710)
rat ... Tuba1a(64158)

Description

General description

Tubulin is a 100kD protein that is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-tubulin, polyglutamylated antibody can be used as primary antibody in colocalization experiments. This antibody can also be used for studies involving the inhibition of flagellar motility in permeabilized sperm models. Mouse anti-tubulin, polyglutamylated antibody reacts specifically with the epitope present in the C-terminal region of α and β tubulins (glutamylated motif at amino acids 445-457 of α-tubulin).

Tubulin is a cellular colchicine-binding protein.

Immunogen

purified sea urchin (Lytechinus pictus) sperm axonemal proteins.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Western Blotting (1 paper)

Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse has been used in:
• immunoprecipitation
• immunohistochemistry
• immunofluorescence

Monoclonal anti-tubulin, polyglutamylated antibody can be used in immunoblotting, immunocytochemistry and ELISA. It can also be used as primary antibodies (diluted 1:1000) in western blotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Microtubule plays an important role in DNA segregation.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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