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V6012 Sigma-Aldrich

Vascular Endothelial Growth Factor D human

recombinant, expressed in baculovirus infected Sf21 cells, powder, suitable for cell culture

Synonym: VEGF-D, c-fos-induced growth factor (FIGF)

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Description

Application

VEGF-D is involved in the regulation of the growth and/or differentiation of lymphatic endothelium and thus, a mitogen for endothelial cells

Biochem/physiol Actions

Vascular Endothelial Growth Factor D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a member of the VEGF family of growth factors. Vascular endothelial growth factor (VEGF) family consists of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGF is a dimeric glycoprotein that stimulates endothelial cells, induces angiogenesis, promotes cell migration, increases vascular permeability and inhibits apoptosis. VEGF-D is most closely related to VEGF-C (23.3% amino acid sequence identity) and has similar VEGF homology domain that spans the middle third of the precursor protein, and long N- and C-terminal extensions.

In adults, VEGF-D is highly expressed in lung, heart, muscle and small intestine. VEGF-D has special relevance in the vascularization of lung tissue during the last trimester of fetal development. Recombinant human VEGF-D is a ligand for the tyrosine kinases, VEGF receptor 2 (Flk1) and VEGF receptor 3 (Flt4). VEGF R3 is strongly expressed in lymphatic endothelial cells and it is postulated that VEGF-D is involved in the regulation of the growth and/or differentiation of lymphatic endothelium and thus, a mitogen for endothelial cells.

Physical form

Lyophilized in phosphate buffered saline containing 1.25 mg bovine serum albumin.

Preparation Note

Recombinant Human Vascular Endothelial Growth Factor D (VEGF-D) is produced from the DNA sequence encoding the VEGF domain of human VEGFD and fused to the signal peptide of CD33 at the N-terminus and to a 6X histidine tag at the C-terminus. The chimeric protein is expressed in Sf 21 cells using a baculovirus expression system. Based on N-terminal sequencing, Met 17 from the CD33 signal peptide is retained in the recombinant protein. This methionyl form contains 115 amino acid residues with a calculated molecular mass of approximately 13 kDa. As a result of glycosylation, the recombinant protein migrates as a 20 and 22 kDa protein in SDS-PAGE under reducing and non-reducing conditions. This recombinant VEGF-D also contains a small amount of disulfide-linked homodimeric VEGF-D.

Analysis Note

The biological activity of VEGF-D is measured by its ability to bind to recombinant human FLT-4/Fc in a ELISA assay.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Vascular Endothelial Growth Factors (VEGF)

Vascular endothelial growth factor (VEGF) is a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. Originally known simpl...
Jennifer Fries
BioFiles 2009, 4.5, 26.
Keywords: Angiogenesis, Gene expression, Growth factors, Inflammation, Ligands

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Peer-Reviewed Papers
15

References

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