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  • X0879 - Anti-XPB antibody produced in rabbit

X0879 Sigma-Aldrich

Anti-XPB antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-BTF2, Anti-ERCC3, Anti-Excision repair cross-complementing rodent repair deficiency, complementation group 3



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to DNA Repair Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 10-20 μg/mL using HeLa cells fixed with paraformaldehyde-Triton
  western blot: 1-2 μg/mL using HepG2 cell lysates
species reactivity   rat, human, mouse
mol wt   antigen 90 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P19447
Gene Information   human ... ERCC3(2071)
mouse ... Ercc3(13872)
rat ... Ercc3(291703)


General description

XPB (Xeroderma pigmentosum complementation group B) is located on the human chromosome at 2q14.3. TFIIH (General transcription and DNA repair factor IIH helicase) consists of nine protein subunits, XPB (ERCC3) being one of them. It is an ATP-dependent DNA helicase of 89 kDa. The XPB is localized in the neurons.


synthetic peptide corresponding to amino acids 765-782 of human XPB, also known as ERCC3, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide differs from the mouse and rat sequences in one and three amino acids respectively.


Anti-XPB antibody has been used in immunoblotting andimmunofluorescence.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

XPB (General transcription and DNA repair factor IIH helicase subunit XPB) is involved in DNA repair and transcription. Phosphorylation on Ser751 of XPB inhibits TFIIH repair activity, leaving its transcription function intact. Mutations in the gene encoding XPB causes Xeroderma Pigmentosum, Cockayne′s syndrome, and trichothiodystrophy.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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