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  • Z370258 - Enzyme Immunoassays: From Concept to Product Development


Enzyme Immunoassays: From Concept to Product Development

  •  ISBN-10 0-412-05601-1

  •  ISBN-13 978-0-412-05601-7



publication info   S. Deshpande, Kluwer Academic, 1996, 288 pp., hard cover
mfr. no.   Kluwer Academic Publishers


General description

A vertical exposition of enzyme immunoassays; the intended audience is product designers and R&Dgroups who aim to commercialize EIA technology. From concepts of antibody and antigen through laboratory development, all the way to manufacturing, marketing, and regulatory compliance, the book is a practical resource for the diagnostic field.

Table of Contents

Part 1: Basic considerations (concepts): Introduction;
Antibody: biochemistry, structure, and function;
Antigen: antibody reactions;
Conjugation techniques;
Antibody production;
Enzymes and signal amplification systems;
Separation and solid phase systems;
Part 2: Product development: Immunoassay classification and commercial technologies;
Assay development, evaluation, and validation;
Reagent formulations and shelf life evaluation;
Data analysis;
Documentation, Registration, and Diagnostic Start-Ups.

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Protocols & Articles


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Keywords: Capture ELISA, Cell culture, Cell signaling, Enzyme-linked immunosorbent assay, Hormones, Indirect ELISA, Titrations

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