Z373672 Sigma-Aldrich


  •  ISBN-10 0-12-214730-8

  •  ISBN-13 978-0-12-214730-2



publication info   E. Diamandis and T. Christopoulus, Academic Press, San Diego, CA, 1996, 579 pp., soft cover
mfr. no.   Elsevier


General description

An indispensable guide to information on the theory and practice of immunoassays. This book balances the content among theory, practical issues, quality control, automation, and subspecialty areas. Protocols for specific assays are included.

Table of Contents

Preface. Past, Present, and Future of Immunological Assays. Immune Function and Antibody Structure. Theory of Immunoassays. Data Interpretation and Quality Control. , Production and Purification of Antibodies. Antibody Engineering. Interferences in Immunoassays. Labeling of Antibodies and Antigens. Solid-Phases in Immunoassay.
Immunoassay Configurations. The Avidin–Biotin System. Radioimmunoassay. Enzyme Immunoassay. Fluorescence Immunoassays. Chemiluminescence Immunoassay. Bioluminescence Immunoassays. Nephelometric and Turbidimetric Immunoassay. Simultaneous Multianalyte
Immunoassays. Noncompetitive Immunoassay for Small Molecules. Free Hormone Measurements. Automation of Immunological Assays. Thin-Film Immunoassays. Immunoblotting Techniques. Development of In-House Immunological Assays. Chapter References. Subject Index.

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Protocols & Articles


ELISA Procedures

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Keywords: Capture ELISA, Cell culture, Cell signaling, Enzyme-linked immunosorbent assay, Hormones, Indirect ELISA, Titrations

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