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53323-U Supelco

Ascentis® Express Peptide ES-C18, 2.7 Micron HPLC Column

2.7 μm particle size, L × I.D. 7.5 cm × 4.6 mm

  •  eCl@ss 32110501



Related Categories Analytical/Chromatography, Ascentis Express Columns for Fast HPLC, Ascentis Express Peptide ES-C18, 2.7μm HPLC Columns, HPLC, HPLC Columns, by Brand for Small Molecules,
feature   endcapped: no
parameter   ≤100 °C temp. range
  600 bar max. pressure (9000 psi)
application(s)   HPLC: suitable
L × I.D.   7.5 cm × 4.6 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
matrix active group   C18 (octadecyl) phase
particle size   2.7 μm
pore size   160 Å
surface area   80 m2/g
operating pH range   1 - 9
suitability   suitable for L1 per USP
mode of chromatography   reversed phase


General description

Ascentis Express Peptide ES-C18 columns are specifically engineered to separate higher molecular weight compounds such as peptides and small proteins. These columns contain advanced Fused-Core particles that have larger pores (160 Å versus 90 Å in standard Ascentis Express), bonded with sterically-protected C18 ligands to provide extra stability (ES) at very low pH (< 1) and high temperatures (up to 100ºC). This greatly expands the application range for Ascentis Express columns.

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Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

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NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Related Content

Selection Guide for Ascentis® Express HPLC Columns

Ascentis Express C18 is the first choice for starting a new method. However, when a C18 doesn't give the desired separation or your sample contains compounds that are known to be difficult to retain ...
Keywords: High performance liquid chromatography, Separation, Supercritical fluid chromatography

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers


Related Products

guard cartridge

Product #


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53542-U Ascentis® Express Peptide ES-C18, 2.7 Micron Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea

required but not provided

Product #


Add to Cart

53500-U Ascentis® Express Guard Cartridge Holder, for use with Ascentis Express Guard Columns, pk of 1

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