• USA Home
  • 53346-U - Ascentis® Express Phenyl-Hexyl, 2.7 Micron HPLC Column

53346-U Supelco

Ascentis® Express Phenyl-Hexyl, 2.7 Micron HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 3 mm



Related Categories Analytical/Chromatography, Ascentis Express Columns for Fast HPLC, Ascentis Express Phenyl-Hexyl, 2.7μm HPLC Columns, HPLC, HPLC Columns, by Brand for Small Molecules,
feature   endcapped
parameter   60 °C temp. range
  600 bar max. pressure (9000 psi)
application(s)   HPLC: suitable
L × I.D.   15 cm × 3 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
matrix active group   phenylhexyl phase
particle size   2.7 μm
pore size   90 Å
pH-range   2 - 9
suitability   suitable for L11 per USP
mode of chromatography   reversed phase


General description

The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

LC-MS Grade Solvents and Reagents

Milli-Q® Water Purification Solutions
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Related Content

Selection Guide for Ascentis® Express HPLC Columns

Ascentis Express C18 is the first choice for starting a new method. However, when a C18 doesn't give the desired separation or your sample contains compounds that are known to be difficult to retain ...
Keywords: High performance liquid chromatography, Separation, Supercritical fluid chromatography

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers


Related Products

guard cartridge

Product #


Add to Cart

53526-U Ascentis® Express Phenyl-Hexyl, 2.7 Micron Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 3 mm, pkg of 3 ea

required but not provided

Product #


Add to Cart

53500-U Ascentis® Express Guard Cartridge Holder, for use with Ascentis Express Guard Columns, pk of 1

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?