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53478-U Supelco

Ascentis® Express ES-Cyano, 2.7 Micron HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 3 mm



Related Categories Analytical/Chromatography, Ascentis Express Columns for Fast HPLC, Ascentis Express ES-Cyano, 2.7μm HPLC Column, ES-Cyano, 2.7μm Columns, HPLC,
feature   endcapped
parameter   ≤80 °C temp. range
  600 bar max. pressure (9000 psi)
application(s)   HPLC: suitable
L × I.D.   5 cm × 3 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
matrix active group   cyano phase
particle size   2.7 μm
pore size   90 Å pore size
pH-range   1 - 8
suitability   suitable for L10 per USP
mode of chromatography   hydrophilic interaction (HILIC)
  normal phase
  reversed phase


General description

Ascentis® Express ES-Cyano HPLC column is a high-speed, high-performance liquid chromatography column based on 90Å Fused-Core® particles. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5-micron thick porous shell and the small overall particle size of 2.7-microns. The sterically protected, extensively endcapped diisopropyl-cyanopropylsilane stationary phase of Ascentis Express ES-Cyano provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Price and Availability

LC-MS Grade Solvents and Reagents

Milli-Q® Water Purification Solutions
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

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Selection Guide for Ascentis® Express HPLC Columns

Ascentis Express C18 is the first choice for starting a new method. However, when a C18 doesn't give the desired separation or your sample contains compounds that are known to be difficult to retain ...
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Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
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Peer-Reviewed Papers


Related Products

guard cartridge

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53496-U Ascentis® Express ES-Cyano, 2.7 Micron Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 3 mm, pkg of 3 ea

required but not provided

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53500-U Ascentis® Express Guard Cartridge Holder, for use with Ascentis Express Guard Columns, pk of 1

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