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53566-U Supelco

Ascentis® Express F5, 2.7 Micron HPLC Column

2.7 μm particle size, L × I.D. 3 cm × 2.1 mm



Related Categories Analytical/Chromatography, Ascentis Express Columns for Fast HPLC, Ascentis Express F5, 2.7μm HPLC Column, F5, 2.7μm Columns, HPLC,
feature   endcapped
parameter   60 °C temp. range
  600 bar max. pressure (9000 psi)
application(s)   HPLC: suitable
L × I.D.   3 cm × 2.1 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
matrix active group   PFP (pentafluorophenyl) phase
particle size   2.7 μm
pore size   90 Å pore size
operating pH range   1 - 9
suitability   suitable for L43 per USP
mode of chromatography   hydrophilic interaction (HILIC)
  reversed phase


General description

The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

LC-MS Grade Solvents and Reagents

Milli-Q® Water Purification Solutions
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Alternative Retention and Selectivity Using Fluorinated Stationary Phases

The use of fluorinated stationary phases in liquid chromatography and hyphenated techniques has become significant in recent years. Early applications in the effective separation of paclitaxel provid...
David S. Bell and Carmen T. Santasania
Reporter US Vol 28.5
Keywords: Anti-inflammatory agents, Applications, Chromatography, Degradations, Environmental, Mass spectrometry, Metabolites, Methods, Pharmaceutical, Phase transitions, Separation, Support

Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Benzodiazepines on Ascentis® Express Phenyl Hexyl HPLC Columns

The vast majority of UHPLC separations have been carried out with C18 columns in the classic reversed-phase (RP) mode; however, suppliers now offer many different phases. Although no one would disput...
Keywords: Buffers, High performance liquid chromatography, Ion Exchange, Phase transitions, Separation, Solvation, Solvents

LC-MS Analysis of Vitamin D Metabolites

Vitamin D deficiency has become a topic of interest in recent publications.1-3 Vitamin D, along with calcium, promotes proper bone growth in children and aids in the prevention of osteoporosis in old...
Craig R. Aurand, David S. Bell
Reporter US Volume 29.2
Keywords: Clinical, Diagnostic, Diseases, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Sample preparations, Separation, Vitamins

Related Content

Boosting HPLC Productivity: Nick the Hero [VIDEO]

Boosting HPLC Productivity Ascentis Express columns provide high-speed and high-efficiency separations that boost lab productivity. Learn about the features and benefits; become the "Nick the Hero" i...
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Selection Guide for Ascentis® Express HPLC Columns

Ascentis Express C18 is the first choice for starting a new method. However, when a C18 doesn't give the desired separation or your sample contains compounds that are known to be difficult to retain ...
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Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
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Related Products

guard cartridge

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53594-U Ascentis® Express F5, 2.7 Micron Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea

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53500-U Ascentis® Express Guard Cartridge Holder, for use with Ascentis Express Guard Columns, pk of 1

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