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53597-U Supelco

Ascentis® Express F5, 2.7 Micron Guard Cartridge

2.7 μm particle size, L × I.D. 5 mm × 3 mm, pkg of 3 ea

  •  eCl@ss 32110501



Related Categories Analytical/Chromatography, Ascentis Express Columns for Fast HPLC, Ascentis Express F5, 2.7μm HPLC Column, F5, 2.7μm Guard Cartridges and Holder, HPLC,
packaging   pkg of 3 ea
application(s)   HPLC: suitable
L × I.D.   5 mm × 3 mm
matrix active group   PFP (pentafluorophenyl) phase
particle size   2.7 μm
pore size   90 Å
suitability   suitable for L43 per USP
mode of chromatography   hydrophilic interaction (HILIC)
  reversed phase


General description

Ascentis Express Guard Columns provide physical (filtration) and chemical protection for costly analytical columns without compromising the very high performance of Ascentis Express columns. These Ascentis Express guard columns are capable of continuous use at pressures up to 9000 psi (600 bar) with only hand-tightening. Guard cartridges are easily replaced without removing the guard column holder from the flow path. The cartridges are packed with Ascentis Express Fused-core® particles. Order guard column holder separately.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Price and Availability

Milli-Q® Water Purification Solutions
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

LC-MS Analysis of Vitamin D Metabolites

Vitamin D deficiency has become a topic of interest in recent publications.1-3 Vitamin D, along with calcium, promotes proper bone growth in children and aids in the prevention of osteoporosis in old...
Craig R. Aurand, David S. Bell
Reporter US Volume 29.2
Keywords: Clinical, Diagnostic, Diseases, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Sample preparations, Separation, Vitamins

Related Content

Selection Guide for Ascentis® Express HPLC Columns

Ascentis Express C18 is the first choice for starting a new method. However, when a C18 doesn't give the desired separation or your sample contains compounds that are known to be difficult to retain ...
Keywords: High performance liquid chromatography, Separation, Supercritical fluid chromatography

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

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53500-U Ascentis® Express Guard Cartridge Holder, for use with Ascentis Express Guard Columns, pk of 1

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