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53829-U Supelco

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, Ascentis Express C18 Columns, Ascentis Express C18, 2.7μm HPLC Columns, Ascentis Express Columns for Fast HPLC, C18, 2.7μm Columns,
Quality Level   100
feature   endcapped
mfr. no.   Ascentis
parameter   60 °C temp. range
  600 bar max. pressure (9000 psi)
application(s)   HPLC: suitable
  UHPLC: suitable
L × I.D.   15 cm × 4.6 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
matrix active group   C18 (octadecyl) phase
particle size   2.7 μm
pore size   90 Å
operating pH   2 - 9
suitability   suitable for L1 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

General description

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

Application

HPLC Analysis of Capsaicins in Chili Powder on Ascentis® Express C18, 2.7 μm
UHPLC/MS Analysis of Corn Oil on Ascentis® Express C30 Compared to Ascentis® Express C18
UHPLC Analysis of Paracetamol and Impurities on Ascentis® Express C18, 2.7 μm
HPLC Analysis of Parabens on Ascentis®Express C18, 2.7 μm

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

UPLC is a registered trademark of Waters

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
WGK Germany 
3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is special about Ascentis Express?
Ascentis Express columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis Express provides the benefits of sub-2 μm particles but at much lower backpressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
When are additional phases expected?
Additional phases are being developed.The best way to track new products is to visitAscentis Express for the latest updates.
Is there anything special I need to do to my HPLC system to use Ascentis Express?
Nothing special is required to use Ascentis Express HPLC columns.To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level.For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).
Can I use Ascentis Express on any type of HPLC system?
Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
Can I use Ascentis Express on a UHPLC system?
Yes.Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How can I measure my instrument bandwidth (IBW) and determine whatAscentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
What flow rate should I use with Ascentis® ExpressHPLC Columns?
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
Can Ascentis® Express HPLC Columns be used for LC-MS?
Express Fused-Core particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
What is the carbon load for Ascentis® Express C18 HPLC Columns?
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared
My question is not addressed here, how can I contact Technical Service for assistance?
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Protocols & Articles

Articles

ASSET EZ4-NCO Analysis of Isocyanates

Introduction Isocyanates are used as a raw material to produce a number of products such as automotive paints, rigid and spray foam insulation, and furniture. Personal exposure can occur while the pr...
Jamie Brown, Emily Barrey, Olga Shimelis and Kristen Schultz
Reporter US Volume 30.3
Keywords: Air sampling, Automotive, Degradations, Derivatizations, Detection methods, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Respiratory, Sample preparations

Ascentis Express C8 U/HPLC Columns

When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column. Th...
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Separation

Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Ascentis ® Express C18 U/HPLC Columns

Ascentis® Express C18 is the first choice when starting a new separation method. The Fused-Core® particle technology of Ascentis® Express C18 enables high speed and high resolution separations on HPL...
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Separation

Assay and Organic Impurity Profiling of Apixaban Using an Ascentis® Express C18 Column and UV Detection

Apixaban is a selective, orally bioavailable, and reversible inhibitor of free and clot-bound factor Xa (which catalyzes the conversion of prothrombin to thrombin). Apixaban is sold under the trade n...
Keywords: High performance liquid chromatography

Assay and Organic Impurity Profiling of Apixaban using an Ascentis Express C18 column and UV Detection

Inject standard solution and determine the retention time of desired analyte in the presence of other components like impurities and excipient.
Keywords: High performance liquid chromatography

Derivatization and Separation of Aliphatic Amines

Hugh Cramer, Associate Scientist Shyam Verma, Market Segment Manager Michael Kiselewsky, Product Manager
AnalytiX, Volume 2, 2011
Keywords: Absorption, Derivatizations, High performance liquid chromatography, Separation

EPA Method 8315: (Solid Waste/Ground Water Carbonyls by HPLC-UV) on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents EPA Method 8315: (Solid Waste/Ground Water Carbonyls by HPLC-UV) on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography

Enlargements of the TIC Chromatogram of the NARP-HPLC-APCI-MS Analysis of Invernizzina Cheese on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents Enlargements of the TIC Chromatogram of the NARP-HPLC-APCI-MS Analysis of Invernizzina Cheese on Ascentis® Express C18
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations, Separation

Fast and Robust HPLC Separation of Bile Acids and Conjugates with Ascentis Express C18

Bile acids are increasingly recognized as playing an important signaling role in the control of immune response and energy metabolism. Recently, there has been interest in measuring bile acid concent...
Clare Glicksman, Michael Wright, Dave Bell, and Anders Fridström
Reporter US, Volume 33.1
Keywords: Bacterial conjugations, Clinical, High performance liquid chromatography, Hormones, Mass spectrometry, Metabolism, Separation, Thin layer chromatography

HPLC Analysis Report of Ginsenosides on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis Report of Ginsenosides on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Aflatoxins on Ascentis® Express C18 with Fluorescence Detection

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Aflatoxins on Ascentis® Express C18 with Fluorescence Detection
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Benzene and Deuterated Benzene on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Benzene and Deuterated Benzene on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Capsacinoids in a Capsicum annuum L. extract.on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Capsacinoids in a Capsicum annuum L. extract.on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Chinese Ginseng Extract on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Chinese Ginseng Extract on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Separation, Vitamins

HPLC Analysis of DNPH Carbonyl Compounds on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of DNPH Carbonyl Compounds on Ascentis® Express C18
Keywords: Air Monitoring, Chromatography, High performance liquid chromatography, Solid phase extractions

HPLC Analysis of Fat-Soluble Vitamins on Ascentis® Express C18, Methanol Mobile Phase

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fat-Soluble Vitamins on Ascentis® Express C18, Methanol Mobile Phase
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Ginsenoside Standards on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Ginsenoside Standards on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Separation, Vitamins

HPLC Analysis of Lansoprazole (Degradation Profile) on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Lansoprazole (Degradation Profile) on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Maté Leaves on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Maté Leaves on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Sample preparations, Vitamins

HPLC Analysis of Triacylglycerols (TAG) of Borago Officinalis Oil on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Triacylglycerols (TAG) of Borago Officinalis Oil on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Mass spectrometry

HPLC Analysis of Triacylglycerols in Sacha Inchi Oil on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Triacylglycerols in Sacha Inchi Oil on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Mass spectrometry

Improving the Current USP Method for the Analysis of Lansoprazole Drug Substance Using HPLC Columns Based on Fused-Core® Particle Technology

Compendial methods from the USP (United States Pharmacopeia) are widely used in pharmaceutical drug product and raw materials testing. However, not all methods in the USP use modern technologies. In ...
Kai Li, Jiajie He, and Xiaoya Ding
PPD - 8551 Research Way, Suite 90, Middleton, WI 53562
Keywords: High performance liquid chromatography, Pharmaceutical, Reductions

LC-MS Determination of Triacylglycerols in Expired Dairy Products and Seafood

Marco Beccaria1 and Luigi Mondello1,2 1Chromaleont s.r.l., c/o “Scienze del Farmaco e Prodotti per la Salute” Department, University of Messina, viale Annunziata, 98168 Messina, Italy 2“Scienze del F...
Reporter US Volume 33.4 (Food & Beverage Supplement)
Keywords: Flame ionization detector, Gas chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Nutrition, Oxidations, Phase transitions, Separation

LC/MS (NARP-LC-APCI(+)-MS) Analysis of Triacylglycerols in Gilthead Bream Extract on Ascentis® Express C18 following Extraction by the Folch Method

From our library of Articles, Sigma-Aldrich presents LC/MS (NARP-LC-APCI(+)-MS) Analysis of Triacylglycerols in Gilthead Bream Extract on Ascentis® Express C18 following Extraction by the Folch Method
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Separation

LC/MS (NARP-LC-APCI(+)-MS) Analysis of Triacylglycerols in Sparus aurata Fractions 3, 4, and 5 Isolated by 1D Silver Ion on Ascentis® Express C18, Mass Scan Number 14,174 (35.43 min)

From our library of Articles, Sigma-Aldrich presents LC/MS (NARP-LC-APCI(+)-MS) Analysis of Triacylglycerols in Sparus aurata Fractions 3, 4, and 5 Isolated by 1D Silver Ion on Ascentis® Express C18, Mass Scan Number 14,174 (35.43 min)
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS (NARP-LC-APCI(+)-MS) Analysis of Triacylglycerols in Sparus aurata on Ascentis® Express C18, Mass Scan Number 14,174 (35.43 min)

From our library of Articles, Sigma-Aldrich presents LC/MS (NARP-LC-APCI(+)-MS) Analysis of Triacylglycerols in Sparus aurata on Ascentis® Express C18, Mass Scan Number 14,174 (35.43 min)
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS Analysis of Bile Acids and Their Conjugates on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Bile Acids and Their Conjugates on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS Analysis of Triacylglycerols (TAG) in Human Plasma on Ascentis® Express C18, NARP-HPLC-APCI-qMS Method

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Triacylglycerols (TAG) in Human Plasma on Ascentis® Express C18, NARP-HPLC-APCI-qMS Method
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Size-exclusion chromatography

LC×LC/MS Method for Identification of Triacylglycerols in Marine Organisms using Different Extraction Procedures

The sea and its living organisms constitute samples of high interest in environmental analysis. Knowledge of the chemistry of the sea and marine species can provide reliable information about polluti...
Marco Beccaria1 and Luigi Mondello1,2

1Chromaleont s.r.l., c/o “Scienze del Farmaco e Prodotti per la Salute” Department, University of Messina, viale Annunziata, 98168 Messina, Italy
2“Scienze del Farmaco e Prodotti per la Salute” Department, University of Messina, viale Annunziata, 98168 Messina, Italy
Keywords: Cardiovascular, Chromatography, Environmental, Environmental Analysis, Gas chromatography, High performance liquid chromatography, Ion-exchange chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Metabolism, Phase transitions, Sample preparations, Separation

Lopinavir European Pharmacopeia HPLC Assay Method

This paper illustrates how it is possible to set-up the assay method for Lopinavir testing following the current European pharmacopeia guidelines (10.2). The monograph assay method calls for a column...
Anita Piper
Merck KGaA, Darmstadt, Germany
Keywords: Chromatography, High performance liquid chromatography, Reductions, Separation

NARP-HPLC-APCI-MS Analysis of TAGs from Invernizzina Cheese on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents NARP-HPLC-APCI-MS Analysis of TAGs from Invernizzina Cheese on Ascentis® Express C18
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations, Separation

NARP-HPLC-APCI-MS Analysis of TAGs from Noccioline Mozzarella Cheese on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents NARP-HPLC-APCI-MS Analysis of TAGs from Noccioline Mozzarella Cheese on Ascentis® Express C18
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations, Separation

NARP-HPLC-APCI-MS Analysis of TAGs from Yogurt on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents NARP-HPLC-APCI-MS Analysis of TAGs from Yogurt on Ascentis® Express C18
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Separation

Rapid Screening of Pharmaceutical Compounds | Fast HPLC

HPLC is critical to the discovery, development and eventual commercialization of pharmaceutical products. HPLC is the benchmark analytical method in the pharmaceutical industry due to its ability to ...
Hillel Brandes
Reporter EU Volume 30
Keywords: Anti-depressants, Chromatography, Diffusion, High performance liquid chromatography, Mass spectrometry, PAGE, Pharmaceutical, Reductions, Separation

Separation of Ginsenosides in Panax Quinquefolius (American Ginseng)

Ginsenosides are mainly found in ginseng plants (genus Panax), widely used as a herbal medicine around the world but primarily in Asian cultures. Most ginsenosides are members of the dammarane family...
Keywords: Chromatography, Diabetes, Nuclear magnetic resonance spectroscopy, Pharmaceutical, Separation, Sonication, Thin layer chromatography

Serum Lipidomics Profiling | Ascentis Express Fused-Core C18 Column

The following was generated with the assistance of an outside source using Sigma-Aldrich products. Technical content was generated and provided by:
Reporter US Volume 29.5
Keywords: Analytical Chemistry, Chromatography, Diffusion, Gas chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Phase transitions, Separation

US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18
Keywords: Chromatography, Environmental, Environmental Analysis, Herbicides, High performance liquid chromatography, Pesticides

US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C8

From our library of Articles, Sigma-Aldrich presents US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C8
Keywords: Chromatography, Environmental, Environmental Analysis, Herbicides, High performance liquid chromatography, Pesticides

US EPA Method 8315 (Solid Waste/Ground Water Carbonyls by HPLC-UV) on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents US EPA Method 8315 (Solid Waste/Ground Water Carbonyls by HPLC-UV) on Ascentis® C18
Keywords: High performance liquid chromatography

Related Content

Ascentis® Express U/HPLC Column Selection

The column selectivity has the highest influence on resolution in chromatography. Therefore, the selection of the best suitable column chemistry for the target analytes is an important selection para...
Keywords: Chromatography, High performance liquid chromatography, Separation

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers
15

References

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53500-U Ascentis® Express Guard Cartridge Holder, for use with Ascentis Express Guard Columns, pk of 1
53508-U Ascentis® Express C18, 2.7 Micron Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea

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