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53912-U Supelco

Ascentis® Express RP-Amide, 2.7 Micron HPLC Column

2.7 μm particle size, L × I.D. 7.5 cm × 2.1 mm



Related Categories Analytical/Chromatography, Ascentis Express Columns for Fast HPLC, Ascentis Express RP-Amide, 2.7μm HPLC Columns, HPLC, HPLC Columns, by Brand for Small Molecules,
feature   endcapped
parameter   60 °C temp. range
  600 bar max. pressure (9000 psi)
application(s)   HPLC: suitable
L × I.D.   7.5 cm × 2.1 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
  Fused-Core® particle platform
matrix active group   amide, alkyl phase
particle size   2.7 μm
pore size   90 Å
operating pH range   2 - 9
suitability   suitable for L60 per USP
mode of chromatography   reversed phase


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Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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LC-MS Grade Solvents and Reagents

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NONH for all modes of transport
WGK Germany 
Protocols & Articles


Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Ascentis® Express RP-Amide HPLC Columns

Ascentis Express RP-Amide HPLC columns are the most recent product additions to the Supelco HPLC product line. Combining an embedded polar group (EPG) stationary phase with the Fused-Core particles, ...
Ascentis® Express HPLC Resource Guide
Keywords: Addition reactions, Anti-depressants, High performance liquid chromatography, Ion Exchange, Ligands, Separation

Synthetic Cannabinoids – New Standards, Methods and Suitable Sample Utilities for Forensic Toxicology

In most countries, the ownership and trade of products from the plant Cannabis sativa, like hashish or marijuana, is illegal, and driving under the influence of cannabinoids can result in prosecution...
Rudolf Köhling
AnalytiX Volume 9 Article 4
Keywords: Adsorption, Cancer, Chromatography, Degradations, Derivatizations, Gas chromatography, High performance liquid chromatography, Mass spectrometry, Metabolites, Pharmaceutical, Phase transitions, Sample preparations, Separation, Solvents

Related Content

Boosting HPLC Productivity: Nick the Hero [VIDEO]

Boosting HPLC Productivity Ascentis Express columns provide high-speed and high-efficiency separations that boost lab productivity. Learn about the features and benefits; become the "Nick the Hero" i...
Keywords: High performance liquid chromatography

Selection Guide for Ascentis® Express HPLC Columns

Ascentis Express C18 is the first choice for starting a new method. However, when a C18 doesn't give the desired separation or your sample contains compounds that are known to be difficult to retain ...
Keywords: High performance liquid chromatography, Separation, Supercritical fluid chromatography

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers


Related Products

guard cartridge

Product #


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53514-U Ascentis® Express RP-Amide, 2.7 Micron Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea

required but not provided

Product #


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53500-U Ascentis® Express Guard Cartridge Holder, for use with Ascentis Express Guard Columns, pk of 1

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