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55234-U Supelco

Supel QuE

Acetate Tube, pk of 50, AOAC® 2007.01

Synonym: QuEChERS

  •  NACRES NB.21

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Properties

Related Categories AOAC 2007.01, Analytical/Chromatography, Sample Preparation & Purification, Solid Phase Extraction (SPE), Supel QuE (QuEChERS) Products More...
Agency/Method   AOAC® 2007.01
Quality Level   100
composition   magnesium sulfate, 6 g
  sodium acetate, 1.5 g (Cat. No. 58750)
packaging   pk of 50
centrifuge tube volume   12 mL , Greiner Cat. No. 163270
Featured Industry   Food and Beverages
separation technique   ion exchange

Description

General description

Dispersive SPE (dSPE), often referred to as the "QuEChERS" method (Quick, Easy, Cheap, Effective, Rugged, and Safe), is modern sample prep technique that is becoming increasingly popular in the area of multi-residue pesticide analysis in food and agricultural products.

Using the QuEChERS method, food/agricultural samples are first extracted with an aqueous miscible solvent (e.g., acetonitrile) in the presence of high amounts of salts (e.g., sodium chloride and magnesium sulfate) and/or buffering agents (e.g. citrate) to induce liquid phase separation and stabilize acid and base labile pesticides, respectively. Upon shaking and centrifugation, an aliquot of the organic phase is subjected to further cleanup using SPE. Unlike traditional methods using SPE tubes, in dispersive SPE, cleanup is facilitated by mixing bulk amounts of SPE (e.g., Supelclean PSA, ENVI-Carb, and/or Discovery DSC-18) with the extract. After sample cleanup, the mixture is centrifuged and the resulting supernatant can either be analyzed directly or can be subjected to minor further treatment before analysis.

Supelco carries a line of vials and centrifuge tubes containing pre-determined amounts of salts and SPE sorbents to support the most common method configurations used today.

Suitability

Suitable for extracting 10-15 g food/agricultural sample. The high level of salts (e.g. magnesium sulfate) is to induce phase separation between the acetonitrile and aqueous sample layer.

Legal Information

AOAC is a registered trademark of AOAC International

Supel is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Banned and Other Neonicotinoids from Plant Material

On Friday, April 27, 2018, representatives of the Member States of the European Union decided to  ban the use of three products from the neonicotinoid family of insecticides as they have been deemed ...
Keywords: High performance liquid chromatography, Insecticides, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Separation

Cleanup of Spinach Extracts Prior to Pesticide Analysis

Prior to analysis for pesticide residues, extracts of highly pigmented foods, such as spinach, require a cleanup step for removal of matrix materials. Failure to do so can lead to ion suppression in ...
Katherine K. Stenerson and Jennifer Claus
Reporter US Volume 31.1
Keywords: Gas chromatography, Gas chromatography mass spectrometry, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Solid phase extractions, Solvents

Determination of Neonicotinoids in Honey using a Chromolith RP-18 HPLC column and UV Detection

Neonicotinoids are water soluble insecticides. They are used for crop protection and can migrate from treated soil into plant tissue and flower nectar. Neonicotinoids have a negative effect on brain ...
Keywords: High performance liquid chromatography, Insecticides, Pesticides, Sample preparations

Extraction of Permethrin Pesticides from Spinach Using QuEChERS Methodology with Automated Shaking

Sample preparation procedures for pesticide analysis in fruits and vegetables were simplified with the introduction of QuEChERS methodology in 20031. Multi-residue pesticide extraction was accomplish...
Olga Shimelis, K. G. Espenschied, Michael Ye, Jennifer Claus
Reporter US Volume 33.1
Keywords: Environmental, Gas chromatography, Mass selective detector, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions, Vitamins

Extraction of Planar Pesticides from Spinach and Fresh Oregano Using QuEChERS Methodology With a Novel Carbon Adsorbent

Although vital to plant life, the presence of chlorophyll in GC/MS analysis is highly problematic. Chlorophyll, a pigment of high molecular weight, is known to quickly cause fouling of injection line...
Michael Ye, and Jennifer Claus
Reporter US Volume 34.2
Keywords: Gas chromatography, Mass spectrometry, Pesticides

Fast Extraction and Low Level Detection of Chloramphenicol in Shrimp

QuEChERS Sample Preparation Using Z-Sep+ Sorbent and Analysis by Ascentis® Express C18 LC-MS/MS
Emily Barrey, Olga Shimelis, Michael Halpenny, Jennifer Claus
Reporter US Volume 32.1
Keywords: Antibiotics, Carcinogens, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Phase transitions, Precipitation, Sample preparations

GC Analysis of Pesticides in Avocado on SLB®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep+, Fast GC Analysis

From our library of Articles, Sigma-Aldrich presents GC Analysis of Pesticides in Avocado on SLB®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep+, Fast GC Analysis
Keywords: Chromatography, Gas chromatography, Gas chromatography mass spectrometry, Mass spectrometry, Pesticides, Purification, Sample preparations, Solid phase extractions

GC Analysis of Pesticides in Beef Kidney on SLB®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel™ Que Z-Sep+, MS in SIM Mode, Fast GC Analysis

From our library of Articles, Sigma-Aldrich presents GC Analysis of Pesticides in Beef Kidney on SLB®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel™ Que Z-Sep+, MS in SIM Mode, Fast GC Analysis
Keywords: Chromatography, Gas chromatography, Gas chromatography mass spectrometry, Mass spectrometry, Pesticides, Purification, Sample preparations, Solid phase extractions

GC Analysis of Pesticides in Grapes on SLB®-5ms after QuEChERS Cleanup using Supel™ QuE PSA/C18

From our library of Articles, Sigma-Aldrich presents GC Analysis of Pesticides in Grapes on SLB®-5ms after QuEChERS Cleanup using Supel™ QuE PSA/C18
Keywords: Chromatography, Gas chromatography, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions

GC Analysis of Pesticides in Spaghetti Sauce on SLB®-5ms after QuEChERS Extraction then Cleanup using Supel QuE PSA/C18

From our library of Articles, Sigma-Aldrich presents GC Analysis of Pesticides in Spaghetti Sauce on SLB®-5ms after QuEChERS Extraction then Cleanup using Supel QuE PSA/C18
Keywords: Chromatography, Gas chromatography, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions

GC Analysis of Pesticides in Spinach on SLB®-5ms after QuEChERS Extraction then SPE Clean-up using Supelclean™ ENVI-Carb/NH2 Dual Layer

From our library of Articles, Sigma-Aldrich presents GC Analysis of Pesticides in Spinach on SLB®-5ms after QuEChERS Extraction then SPE Clean-up using Supelclean™ ENVI-Carb/NH2 Dual Layer
Keywords: Chromatography, Gas chromatography, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions

GC Analysis of Pesticides in Spinach on SLB®-5ms after QuEChERS Extraction then SPE Clean-up using Supel™ Sphere Carbon/NH2

From our library of Articles, Sigma-Aldrich presents GC Analysis of Pesticides in Spinach on SLB®-5ms after QuEChERS Extraction then SPE Clean-up using Supel™ Sphere Carbon/NH2
Keywords: Chromatography, Filtration, Gas chromatography, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions

Improve Recovery for Analysis of Pesticides in Beef Kidney

The exposure of farm animals to agricultural pesticides continues to be a major concern among food producers. For example, in the case of organochlorine pesticides, although their use has been severe...
Katherine K. Stenerson and Jennifer Claus
Reporter US Volume 31.1
Keywords: Agriculture, Chromatography, Fungicides, Gas chromatography, Gas chromatography mass spectrometry, Insecticides, Mass spectrometry, Pesticides, Phase transitions, Sample preparations, Size-exclusion chromatography, Solid phase extractions, Solvents

Increase Removal of Fat & Pigments From Avocado Prior to GC-MS Analysis of Pesticide and Metabolite Residues

The health benefits of avocado are associated with its high content of healthy fat, fiber, vitamins, and minerals. Avocado consumption in the United States has steadily grown, with sources of the fru...
Katherine K. Stenerson and Jennifer Claus
Reporter US Volume 31.2
Keywords: Adsorption, Gas chromatography, Gas chromatography mass spectrometry, Gravimetric analysis, High performance liquid chromatography, Mass spectrometry, Pesticides, Solid phase extractions, Vitamins

LC/MS/MS Analysis of Neonicotinoid Pesticides in Dandelion Blossoms on Ascentis® Express C18 after Dispersive SPE (QuEChERS) using Supel™ QuE

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Neonicotinoid Pesticides in Dandelion Blossoms on Ascentis® Express C18 after Dispersive SPE (QuEChERS) using Supel™ QuE
Keywords: Catalytic combustion detector, Chromatography, Herbicides, High performance liquid chromatography, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions

QuEChERS Extraction and LC-MS Analysis of Neonicotinoid Pesticides.

Introduction Neonicotinoids are systemic insecticides that are toxic to insects but do not affect mammals. They are water soluble and have the ability to migrate from treated soil into plant tissue, ...
Katherine K. Stenerson
Reporter US, Volume 32.1
Keywords: Adsorption, Catalytic combustion detector, Chromatography, High performance liquid chromatography, Insecticides, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Pesticides, Separation

QuEChERS Method for Pesticide Residue Analysis

QuEChERS significantly improves laboratory efficiency and throughput. This procedure requires only small quantities of solvent and is capable of generating recoveries of 70-120% with RSDs <5% for a w...
Keywords: Buffers, Calorimetry, Gas chromatography, Gas chromatography mass spectrometry, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides

Reduce Matrix Background and Improve Overall Analyte Recovery

The exposure of farm animals to agricultural pesticides continues to be a major concern among food producers. For example, in the case of organochlorine pesticides, although their use has been severe...

Solid Phase Extraction: Ion Exchange Methodology

In order for electrostatic retention to occur, both analyte and sorbent functional groups must be in their ionized form. This is done through strict pH control of the sample matrix. For basic analyte...
Keywords: Agriculture, Buffers, Herbicides, Ion Exchange, Pharmaceutical, Solid phase extractions

Solid Phase Extraction: Normal Phase Methodology

In order for polar retention to occur between the sorbent and the sample, the analyte must be introduced to the SPE device in a non-polar sample or mobile phase environment. Therefore, typical sample...
Keywords: Cosmetics, Fractionation, Gas chromatography, Separation, Solid phase extractions, Solvents, Sonication

Related Content

SPE Cartridges (Tubes) - Phase Selection Guide

The SPE Tube Configuraton Guide will help you choose the appropriate bed weight and cartridge dimensions.
Keywords: Agriculture, Calorimetry, Environmental, Fractionation, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Pharmaceutical, Solid phase extractions

Solid Phase Extraction: Fundamentals and Recent Developments [Webinar]

Solid Phase Extraction (SPE) has become a popular technique for sample preparation due to its convenience, effectiveness, relatively low solvent usage and versatility. The focus of this webinar is on...
Keywords: Sample preparations, Solid phase extractions

Peer-Reviewed Papers
15

References

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