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55248-U Supelco

Supel QuE

Empty Centrifuge Tube with Lid, centrifuge tube volume 50 mL, pk of 50, suitable for EN 15662:2008 per BS

Synonym: QuEChERS

  •  NACRES NB.21

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Properties

Related Categories AOAC 2007.01, Analytical/Chromatography, EN 15662, Empty 50 mL Tubes for Extraction, Sample Preparation & Purification,
Agency/Method   AOAC® 2007.01
Quality Level   100
composition   empty tube (n/a)
packaging   pk of 50
centrifuge tube volume   50 mL
suitability   suitable for EN 15662:2008 per BS
Featured Industry   Food and Beverages

Description

General description

Dispersive SPE (dSPE), often referred to as the "QuEChERS" method (Quick, Easy, Cheap, Effective, Rugged, and Safe), is modern sample prep technique that is becoming increasingly popular in the area of multi-residue pesticide analysis in food and agricultural products.

Using the QuEChERS method, food/agricultural samples are first extracted with an aqueous miscible solvent (e.g., acetonitrile) in the presence of high amounts of salts (e.g., sodium chloride and magnesium sulfate) and/or buffering agents (e.g. citrate) to induce liquid phase separation and stabilize acid and base labile pesticides, respectively. Upon shaking and centrifugation, an aliquot of the organic phase is subjected to further cleanup using SPE. Unlike traditional methods using SPE tubes, in dispersive SPE, cleanup is facilitated by mixing bulk amounts of SPE (e.g., Supelclean PSA, ENVI-Carb, and/or Discovery DSC-18) with the extract. After sample cleanup, the mixture is centrifuged and the resulting supernatant can either be analyzed directly or can be subjected to minor further treatment before analysis.

Supelco carries a line of vials and centrifuge tubes containing pre-determined amounts of salts and SPE sorbents to support the most common method configurations used today.

Application

Supel QuE is used as the centrifuge tube for the quick, easy, cheap, effective, rugged, safe (QuEChERS) extraction and analysis of polycyclic aromatic hydrocarbons in foods using gas chromatography coupled to mass spectrometry.

Legal Information

AOAC is a registered trademark of AOAC International

Supel is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Analysis of Active Cannabis Compounds (Cannabinoids) in Edible Food Products: Gummy Bears and Brownies

Potency testing in marijuana-infused edibles is an important problem that analytical labs are facing due to the complexity of the involved matrices. Among the most popular matrices are gummy bear can...
Olga Shimelis,1 Principle R&D Scientist; Kathy Stenerson,1 Principle R&D Scientist; Margaret Wesley,2 2016 R&D Summer Intern
1MilliporeSigma, Bellefonte, PA, 2Pennsylvania State University, University Park, State College, PA, Reporter US Volume 34.4
Keywords: High performance liquid chromatography, Separation

Analysis of Active Cannabis Compounds in Edible Food Products: Gummy Bears and Brownies

Potency testing in marijuana-infused edibles is a problematic task that analytical labs are facing due to the complexity of the involved matrices. Among the most popular matrices are gummy bear candi...
Keywords: High performance liquid chromatography, Sample preparations, Separation

Analysis of Pesticide Residues in Cannabis Using QuEChERS Extraction and Cleanup Followed by GC/MS/MS Analysis

Cannabis (marijuana) has been legalized for recreational and/or medical use by 23 states, the District of Columbia, and the territory of Guam. Thus, regulations regarding its production have become a...
Kathy Stenerson, and Michael Halpenny
Reporter US Volume 34.1
Keywords: Capillary electrophoresis, Fungicides, Gas chromatography, Insecticides, Mass spectrometry, Pesticides, Sample preparations

Analysis of Pesticide Residues in Ginger Powder Using QuEChERS Extraction and Clean-up with a Novel Dual Layer SPE Cartridge

Ginger comes from the rhizome of the plant Zingiber officinale. It has been used for thousands of years as both a spice for cooking and a treatment for ailments such as nausea, gastrointestinal (GI) ...
Keywords: Capillary electrophoresis, Cardiovascular, Enzyme activity, Gas chromatography, Gas chromatography mass spectrometry, Gastrointestinal, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass selective detector, Mass spectrometry, Pesticides, Reductions, Solid phase extractions

Analysis of Pesticide Residues in Pistachios – Using QuEChERS Extraction and Cleanup with Supel™ QuE Z-Sep+

Pistachios are popular and enjoyed for both taste and health benefits such as decreased cholesterol, weight management, protection against diabetes and hypertension, and improved digestion.1 These nu...
Keywords: Capillary electrophoresis, Diabetes, Digestions, Gas chromatography, Gas chromatography mass spectrometry, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass selective detector, Mass spectrometry, Pesticides

Analysis of Pesticides in Black Tea by GC/MS/MS on SLB® Using Supelclean™ Ultra Cleanup

From our library of Articles, Sigma-Aldrich presents Analysis of Pesticides in Black Tea by GC/MS/MS on SLB® Using Supelclean™ Ultra Cleanup
Keywords: Gas chromatography, Mass spectrometry, Sample preparations

Analysis of Pesticides in Turmeric Powder by LC/MS/MS and GC/MS/MS after Cleanup with a Novel Dual-Layer SPE Cartridge

Turmeric is a plant indigenous to south Asia, with a majority of its production coming from India. The rhizome of the plant is used to produce powdered turmeric, which is used in foods, cosmetics, an...
Katherine K. Stenerson
Principal R&D Scientist
Keywords: Anti-inflammatory agents, Antimicrobials, Capillary electrophoresis, Cosmetics, Gas chromatography, High performance liquid chromatography, Mass selective detector, Mass spectrometry, Pesticides, Reductions, Solid phase extractions

Cleanup of Spinach Extracts Prior to Pesticide Analysis

Prior to analysis for pesticide residues, extracts of highly pigmented foods, such as spinach, require a cleanup step for removal of matrix materials. Failure to do so can lead to ion suppression in ...
Katherine K. Stenerson and Jennifer Claus
Reporter US Volume 31.1
Keywords: Gas chromatography, Gas chromatography mass spectrometry, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Solid phase extractions, Solvents

Determination of Neonicotinoids in Honey using a Chromolith RP-18 HPLC column and UV Detection

Neonicotinoids are water soluble insecticides. They are used for crop protection and can migrate from treated soil into plant tissue and flower nectar. Neonicotinoids have a negative effect on brain ...
Keywords: High performance liquid chromatography, Insecticides, Pesticides, Sample preparations

Determination of PAHs in Paprika Powder Using a Novel SPE Cartridge

Paprika is a spice made from dried sweet peppers, and is used for flavor and color in many types of cuisine. Contamination with polynuclear aromatic hydrocarbons (PAHs) can occur when pepper plants a...
Katherine K. Stenerson
Keywords: Capillary electrophoresis, Chromatography, Gas chromatography, Mass spectrometry, Pesticides, Reductions, Sample preparations, Size-exclusion chromatography, Solid phase extractions, Solvents

Extraction of Permethrin Pesticides from Spinach Using QuEChERS Methodology with Automated Shaking

Sample preparation procedures for pesticide analysis in fruits and vegetables were simplified with the introduction of QuEChERS methodology in 20031. Multi-residue pesticide extraction was accomplish...
Olga Shimelis, K. G. Espenschied, Michael Ye, Jennifer Claus
Reporter US Volume 33.1
Keywords: Environmental, Gas chromatography, Mass selective detector, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions, Vitamins

Extraction of Planar Pesticides from Spinach and Fresh Oregano Using QuEChERS Methodology With a Novel Carbon Adsorbent

Although vital to plant life, the presence of chlorophyll in GC/MS analysis is highly problematic. Chlorophyll, a pigment of high molecular weight, is known to quickly cause fouling of injection line...
Michael Ye, and Jennifer Claus
Reporter US Volume 34.2
Keywords: Gas chromatography, Mass spectrometry, Pesticides

Fast Extraction and Low Level Detection of Chloramphenicol in Shrimp

QuEChERS Sample Preparation Using Z-Sep+ Sorbent and Analysis by Ascentis® Express C18 LC-MS/MS
Emily Barrey, Olga Shimelis, Michael Halpenny, Jennifer Claus
Reporter US Volume 32.1
Keywords: Antibiotics, Carcinogens, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Phase transitions, Precipitation, Sample preparations

GC Analysis of PCBs in Whole Cow Milk (Spiked) on SLB®-5ms (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis

From our library of Articles, Sigma-Aldrich presents GC Analysis of PCBs in Whole Cow Milk (Spiked) on SLB®-5ms (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis
Keywords: Chromatography, Electron capture detector, Gas chromatography, Purification, Sample preparations, Solid phase extractions

GC Analysis of Pesticide Residues in Cannabis on SLB®-5ms after QuEChERS Extraction then QuEChERS Cleanup using Supel™ QuE Verde

From our library of Articles, Sigma-Aldrich presents GC Analysis of Pesticide Residues in Cannabis on SLB®-5ms after QuEChERS Extraction then QuEChERS Cleanup using Supel™ QuE Verde
Keywords: Adsorption, Chromatography, Gas chromatography, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions

GC Analysis of Pesticide Residues in Hops on SLB®-5ms after QuEChERS Extraction then QuEChERS Cleanup using Supel™ QuE Verde

From our library of Articles, Sigma-Aldrich presents GC Analysis of Pesticide Residues in Hops on SLB®-5ms after QuEChERS Extraction then QuEChERS Cleanup using Supel™ QuE Verde
Keywords: Adsorption, Chromatography, Gas chromatography, High performance liquid chromatography, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions

GC Analysis of Pesticides in Grapes on SLB®-5ms after QuEChERS Cleanup using Supel™ QuE PSA/C18

From our library of Articles, Sigma-Aldrich presents GC Analysis of Pesticides in Grapes on SLB®-5ms after QuEChERS Cleanup using Supel™ QuE PSA/C18
Keywords: Chromatography, Gas chromatography, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions

GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Grilled Hamburger on SPB®-608 (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis

From our library of Articles, Sigma-Aldrich presents GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Grilled Hamburger on SPB®-608 (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis
Keywords: Chromatography, Gas chromatography, Gas chromatography mass spectrometry, Mass spectrometry, Purification, Sample preparations, Solid phase extractions

GC/MS/MS Analysis of Pesticides in Chamomile on SLB®-5ms after QuEChERS Cleanup usingSupelClean™ Ultra

From our library of Articles, Sigma-Aldrich presents GC/MS/MS Analysis of Pesticides in Chamomile on SLB®-5ms after QuEChERS Cleanup usingSupelClean™ Ultra
Keywords: Gas chromatography, Mass spectrometry, Pesticides, Size-exclusion chromatography, Solid phase extractions

GC/MS/MS Analysis of Pesticides in Ginseng on SLB®-5ms after QuEChERS Cleanup usingSupelClean™ Ultra

From our library of Articles, Sigma-Aldrich presents GC/MS/MS Analysis of Pesticides in Ginseng on SLB®-5ms after QuEChERS Cleanup usingSupelClean™ Ultra
Keywords: Gas chromatography, Mass spectrometry, Pesticides, Size-exclusion chromatography, Solid phase extractions

HPLC Analysis of Active Cannabinoid Compounds in Gummy Bear Candy on Ascentis® Express Biphenyl with UV Detection after Extraction

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Active Cannabinoid Compounds in Gummy Bear Candy on Ascentis® Express Biphenyl with UV Detection after Extraction
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

Improve Analysis of Pesticides in Oranges

Although pesticides have proven to be invaluable in crop control, the same toxicity, stability, and mobility that enables them to effectively kill insects causes detrimental effects in humans and the...
Olga Shimelis, Emily Barrey, and Jennifer Claus
Reporter US Volume 30.3
Keywords: Agriculture, Environmental, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions

Improve Recovery for Analysis of Pesticides in Beef Kidney

The exposure of farm animals to agricultural pesticides continues to be a major concern among food producers. For example, in the case of organochlorine pesticides, although their use has been severe...
Katherine K. Stenerson and Jennifer Claus
Reporter US Volume 31.1
Keywords: Agriculture, Chromatography, Fungicides, Gas chromatography, Gas chromatography mass spectrometry, Insecticides, Mass spectrometry, Pesticides, Phase transitions, Sample preparations, Size-exclusion chromatography, Solid phase extractions, Solvents

Improved Recoveries and Lower Background for GC/MS/MS and LC/MS/MS Analysis of Pesticides in Green Tea

Tea is second only to water as the most popular beverage worldwide. All teas come from the leaves of the same plant, camellia sinensis. The various types of tea arise from differences in how and wher...
Katherine K. Stenerson
Reporter US Volume 34.4
Keywords: Agriculture, Capillary electrophoresis, Gas chromatography, High performance liquid chromatography, Mass spectrometry, Oxidations, Pesticides, Reductions, Solid phase extractions, Vitamins

Improved Recovery of Fungicide Carbendazim from Orange Juice

The fungicide carbendazim can sometimes be used to combat mold on citrus fruits including oranges. Recently, low residue levels of the fungicide were reported to be present in bulk orange juice impor...
Olga Shimelis and Emily Barrey
Reporter US Volume 30.2
Keywords: Chromatography, Fungicides, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations

LC/MS/MS Analysis of Carbendazim in Orange Juice (Spiked) on Ascentis® Express C18 after Supel™ QuE PSA/C18 QuEChERS Cleanup

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Carbendazim in Orange Juice (Spiked) on Ascentis® Express C18 after Supel™ QuE PSA/C18 QuEChERS Cleanup
Keywords: Chromatography, Column chromatography, Fungicides, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations, Solid phase extractions

LC/MS/MS Analysis of Pesticide Residues in Dates on the Ascentis® Express RP-Amide Column after QuEChERS Extraction

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Pesticide Residues in Dates on the Ascentis® Express RP-Amide Column after QuEChERS Extraction
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions

LC/MS/MS Analysis of Pesticide Residues in Pistachios on the Ascentis® Express RP-Amide Column after QuEChERS Extraction

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Pesticide Residues in Pistachios on the Ascentis® Express RP-Amide Column after QuEChERS Extraction
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions

LC/MS/MS Analysis of Pesticides in Cannabis on the Ascentis RP-Amide after QuEChERS Extraction and Cleanup Using Supel QuE Verde

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Pesticides in Cannabis on the Ascentis RP-Amide after QuEChERS Extraction and Cleanup Using Supel QuE Verde
Keywords: High performance liquid chromatography, Mass spectrometry, Pesticides, Reductions

LC/MS/MS Method for Determination of Glyphosate, AMPA, and Glufosinate in Cereals

Glyphosate is one of the most commonly used herbicides in the world. Its usage has significantly increased after the introduction of genetically modified glyphosate tolerant crops such as corn, soybe...
Olga I. Shimelis, Principal R&D Scientist
Reporter US Volume 34.4
Keywords: Absorption, Centrifugation, Derivatizations, Herbicides, High performance liquid chromatography, Mass spectrometry, Metabolites, Pesticides, Phase transitions, Sample preparations, Solid phase extractions

QuEChERS Cleanup and Analysis of Veterinary Drugs in Milk by LC/MS/MS

The use of mass spectrometry (MS) as an analytical technique allows quick screening, identification, and confirmation of contaminants. In spite of advances that MS instrumentation brings to achieve e...
Olga I. Shimelis, and Jennifer Claus
Reporter US Volume 34.1
Keywords: Antibiotics, Calorimetry, Environmental, High performance liquid chromatography, Mass spectrometry, Pesticides, Phase transitions, Sample preparations, Separation, Solid phase extractions, Veterinary

QuEChERS Extraction and LC-MS Analysis of Neonicotinoid Pesticides.

Introduction Neonicotinoids are systemic insecticides that are toxic to insects but do not affect mammals. They are water soluble and have the ability to migrate from treated soil into plant tissue, ...
Katherine K. Stenerson
Reporter US, Volume 32.1
Keywords: Adsorption, Catalytic combustion detector, Chromatography, High performance liquid chromatography, Insecticides, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Pesticides, Separation

Reduce Matrix Background and Improve Overall Analyte Recovery

The exposure of farm animals to agricultural pesticides continues to be a major concern among food producers. For example, in the case of organochlorine pesticides, although their use has been severe...

Related Content

SPE Cartridges (Tubes) - Phase Selection Guide

The SPE Tube Configuraton Guide will help you choose the appropriate bed weight and cartridge dimensions.
Keywords: Agriculture, Calorimetry, Environmental, Fractionation, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Pharmaceutical, Solid phase extractions

Solid Phase Extraction: Fundamentals and Recent Developments [Webinar]

Solid Phase Extraction (SPE) has become a popular technique for sample preparation due to its convenience, effectiveness, relatively low solvent usage and versatility. The focus of this webinar is on...
Keywords: Sample preparations, Solid phase extractions

Peer-Reviewed Papers
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