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  • 568520-U - Discovery® HS C18 HPLC Column

568520-U Supelco

Discovery® HS C18 HPLC Column

5 μm particle size, L × I.D. 5 cm × 4.6 mm

  •  eCl@ss 32110501

  •  NACRES SB.52



Related Categories Analytical/Chromatography, C18 Columns, 5 micron, Discovery HPLC Columns, Discovery HS C18, HPLC Columns, by Brand for Small Molecules,
Quality Level   100
feature   endcapped
mfr. no.   Discovery
extent of labeling   20% Carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   5 cm × 4.6 mm
surface coverage   3.2 μmol/m2
impurities   <10 ppm metals
matrix   silica gel, high purity, spherical particle platform
matrix active group   C18 (octadecyl) phase
particle size   5 μm
pore size   120 Å
surface area   300 m2/g
operating pH range   2 - 8
suitability   suitable for L1 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase


Features and Benefits

• Stable, low bleed for LC-MS applications
• Scalable from analytical to preparatory
• Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility
• Higher hydrophobicity for better resolution of difficult analytes

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Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis (COA)

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Protocols & Articles


Determination of Hydrocortisone from Topical Cream Using Discovery DSC-Si SPE and Reversed-Phase HPLC-UV

Hydrocortisone (Figure A) is a steroid hormone secreted by the adrenal cortex and synthesized for medicinal use. It is a moderately polar compound that contains a glucocorticoid structure (steroid nu...
Keywords: Calorimetry, Chromatography, High performance liquid chromatography, Hormones, Pharmaceutical, Precipitation, Sample preparations, Separation, Solid phase extractions, Thin layer chromatography

HPLC Analysis of Hydrocortisone in Topical Cream on Discovery® HS C18 after SPE using Discovery® DSC-Si

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Hydrocortisone in Topical Cream on Discovery® HS C18 after SPE using Discovery® DSC-Si
Keywords: Calorimetry, Chromatography, Clinical, Cosmetics, High performance liquid chromatography, Pharmaceutical, Sample preparations, Solid phase extractions

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers


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568573-U Discovery® HS C18 Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 4 mm

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