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  • 76704 - mTSB Broth with Novobiocin

76704 Millipore

mTSB Broth with Novobiocin

for microbiology

Synonym: Modified Tryptic Soy Broth with Novobiocin

  •  NACRES NA.85



Related Categories A - M, All Food Control Media (alphabetical), All Liquid Media/Broths, Enterovirulent E. coli, Escherichia coli & Coliforms,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited, expiry date on the label
composition   bile salts No. 3, 1.5 g/L
  di-potassium hydrogen phosphate, 4.0 g/L
  D(+)-glucose, 2.5 g/L
  novobiocin, 0.02 g/L
  peptone from casein, 17.0 g/L
  peptone from soymeal, 3.0 g/L
  sodium chloride, 5.0 g/L
final pH   7.3±0.2 (25 °C)
Featured Industry   Agriculture
Food and Beverages
suitability   selective for Escherichia coli
  selective for coliforms
  selective for enterovirulent E. coli (EEC)



mTSB Broth with Novobiocin has been used for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods. The medium is used for the isolation and identification of Escherichia coli 0157:H7 from meat acc. the USDA-FSIS method.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


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Protocols & Articles


Detection of enterovirulent Escherichia coli

E. coli are Gram-negative, rod-shaped bacteria belonging to the family of Enterobacteriaceae. Normally a useful organism in the human digestive system, a few E. coli strains are capable of causing hu...
Jvo Siegrist
AnalytiX Volume 8 Article 4
Keywords: Clinical, Environmental, Indicators, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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