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159220 Sigma-Aldrich

Diethyl pyrocarbonate

97%

Synonym: DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride

  • CAS Number 1609-47-8

  • Linear Formula O(COOC2H5)2

  • Molecular Weight 162.14

  •  Beilstein/REAXYS Number 637031

  •  EC Number 216-542-8

  •  MDL number MFCD00009106

  •  PubChem Substance ID 24849799

  •  NACRES NA.22

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Properties

Related Categories Alternative Energy, Building Blocks, Carbonates, Carbonyl Compounds, Chemical Synthesis,
assay   97%
refractive index   n20/D 1.398 (lit.)
bp   93-94 °C/18 mmHg (lit.)
density   1.101 g/mL at 25 °C (lit.)
shipped in   wet ice
storage temp.   2-8°C
SMILES string   CCOC(=O)OC(=O)OCC
InChI   1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
InChI key   FFYPMLJYZAEMQB-UHFFFAOYSA-N

Description

General description

Diethyl pyrocarbonate reacts selectively with the single-stranded loops of cruciform structures. The irreversible inactivation of ribonuclease with diethylpyrocarbonate has been studied.

Application

Diethyl pyrocarbonate was used as:
• nuclease inhibitor for the extraction of undegraded nucleic acids from etiolated and green plant tissues
• an antimicrobial additive
• ribonuclease inhibitor
• histidine residue modifier
• reagent for conversion of imines to carbamates

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Packaging

25, 100 g in poly bottle

5 g in glass bottle

Biochem/physiol Actions

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
WGK Germany 
WGK 3
RTECS 
LQ9350000
Flash Point(F) 
156.2 °F - closed cup
Flash Point(C) 
69 °C - closed cup

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Complex Hydrides: A New Category of Solid-state Lithium Fast-ion Conductors

1Institute for Materials Research, Tohoku University Katahira 2-1-1, Sendai, 980-8577, Japan 2Graduate School of Engineering, Tohoku University Aramaki Aza Aoba 6-6-02, Sendai, 980-8579, Japan
Motoaki Matsuo,1 Hiroyuki Oguchi,1,2 Hideki Maekawa,2 Hitoshi Takamura,2Shin-ichi Orimo,1
Material Matters Volume 5 Article 4
Keywords: Calorimetry, Deposition, Diffraction, Diffusion, Melting, Nuclear magnetic resonance spectroscopy, Raman spectroscopy, Spectroscopy, Substitutions, X-Ray diffraction, transformation

Peer-Reviewed Papers
15

References

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