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160539 Aldrich

Ethidium bromide

95%

Synonym: 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide

  • CAS Number 1239-45-8

  • Empirical Formula (Hill Notation) C21H20BrN3

  • Molecular Weight 394.31

  •  Beilstein Registry Number 3642536

  •  EC Number 214-984-6

  •  MDL number MFCD00011724

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Properties

InChI Key   ZMMJGEGLRURXTF-UHFFFAOYSA-N
assay   95%
mp   260-262 °C (dec.)(lit.)
absorption   λmax 506 nm

Description

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Reconstitution

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Frameshift mutagen which intercalates double-stranded DNA and RNA.

Frameshift mutagen which intercalates double-stranded DNA and RNA.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811PIH 6.1 / PGI
WGK Germany 
3
RTECS 
SF7950000
Protocols & Articles
Peer-Reviewed Papers
15

References

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01494 Nancy-520, suitable for RT-PCR, ≥97.0% (HPCE)

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E8751 Ethidium bromide, ~95% (HPLC)

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