198285 Sigma-Aldrich


certified by the Biological Stain Commission

Synonym: Acid black 2, Nigrosin water soluble



Related Categories Cell Biology, Certified Stains & Dyes, Hematology and Histology, N, Stains & Dyes, A to Z,
grade   certified by the Biological Stain Commission
solubility   H2O: soluble
absorption   λmax 570 nm



25, 100 g in glass bottle


Certified for use in Dorner′s spore stain. Used as a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi.

Used as a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Certificate of Origin


Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the solubility ofProduct 198285, Nigrosin, in water?
The maximum solubility in water can vary from 5 to 10%, depending upon the lot number.
What is the purity ofProduct 198285, Nigrosin, Water Soluble?
No dye purity is determined because the dye is a complex mixture of dyes with an unknown constitution.
What is the molecular weight for Product 198285, Nigrosin?
No formula weight or empirical formula is available. The dye is innately a mixture of dyes.
Is Product 198285, Nigrosin, soluble in ethanol?
Solubility is stated to be only 0.0 to 0.4% in ethanol.
Has Product 198285, Nigrosin, been used to test for sperm viability?
Yes. 10% Nigrosin Water Soluble is often used with 5% aqueous Eosin Y to determine live versus dead sperm. A procedure is given in Kjeldsberg, CR and Knight JA; Body Fluids, Second Edition (ASCP Press, Chicago 1986), p 168.
During the certification testing for Product 198285, Nigrosin, what organism is used for testing?
The exact organism can vary, but typically a 2-4 day old culture of a rapid spore former such as Bacillus cereus is utilized.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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