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199583 Sigma-Aldrich

Lissamine Green B

Dye content 60 %

Synonym: Acid Green 50, Wool Green S

  • CAS Number 3087-16-9

  • Empirical Formula (Hill Notation) C27H25N2NaO7S2

  • Molecular Weight 576.62

  •  Colour Index Number 44090

  •  EC Number 221-409-2

  •  MDL number MFCD00012117

  •  PubChem Substance ID 24851928



Related Categories Cell Biology, Hematology and Histology, J-K-L, Stains & Dyes, A to Z, Stains and Dyes More...
composition   Dye content, 60%
absorption   λmax 633 nm



25 g in glass bottle


Lissamine Green B has been used in blood-brain barrier (BBB) studies and is used to study the its effect on herpes simplex virus 1 (HSV-1) corneal infection in animals.

Biochem/physiol Actions

Lissamine green also referred to as acid green 50, wool green S, food green S or green S, is used for vital staining of cornea and conjunctiva. Staining characteristics are almost similar to rose bengal dye. Lissamine green is also used to stain membrane-damaged epithelial cells. In vitro, Lissamine green B at low concentration blocks herpes simplex virus 1 (HSV-1) plaque formation.

Legal Information

Lissamine is a trademark of PerkinElmer Inc.

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the ?max199583, Lissamine Green B, in water?
The ?max in water is 633 nm.
What is the solubility of Product 199583, Lissamine Green B?
Lissamine Green B is soluble in water up to 0.1% in water. Other sources have stated solubility for some lots can approach 4%. Solubility in ethanol is 3 mg/mL, although this is not tested by Sigma Aldrich on a lot to lot basis. Lissamine Green B has also been reported to be soluble in Cellosolve, and in ethylene glycol.
What are possible uses for Product 199583, Lissamine Green B?
The Sigma Aldrich Handbook of Stains, Dyes and Indicators advises that Lissamine Green B has been substituted for Fast Green FCF without any real advantage. Lillie found it performed well as a collagen stain in Masson's trichrome and as a counter stain in the Van Gieson method. However, it was inferior when used as a component in Van Gieson's Trichrome Stain. See also Clark G, Staining Procedures, 4th Edition. (Williams & Wilkins, Baltimore, 1981), p. 120 for the Lillie modification of Masson's trichrome method.This material is used in the determination of chlorine dioxide in solution in EPA Method 32.
HasProduct 199583, Lissamine Green B, been used for industrial applications?
The Sigma Aldrich Handbook of Stains, Dyes and Indicators states Lissamine Green B has been used to dye wool, silk (from a sulfuric acid bath), to print wool and silk directly and to coat paper, dye leather and surface stain wood.
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