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201650 Sigma-Aldrich

Fluorescamine

98%

Synonym: 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione

  • CAS Number 38183-12-9

  • Empirical Formula (Hill Notation) C17H10O4

  • Molecular Weight 278.26

  •  Beilstein/REAXYS Number 921143

  •  EC Number 253-814-5

  •  MDL number MFCD00005928

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Properties

assay   98%
mp   153-157 °C (lit.)
λmax   306 nm
SMILES string   O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14
InChI   1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
InChI key   ZFKJVJIDPQDDFY-UHFFFAOYSA-N

Description

Application

Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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Description

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F9015 Fluorescamine, ≥98% (TLC), powder, used for detection of primary amines

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