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202096 Sigma-Aldrich

New Methylene Blue N zinc chloride double salt

Dye content 90 %

Synonym: Basic blue 24, Methylene blue N

  • CAS Number 6586-05-6

  • Empirical Formula (Hill Notation) C18H22ClN3S · 0.5ZnCl2

  • Molecular Weight 416.05

  •  Colour Index Number 52030

  •  EC Number 229-516-6

  •  MDL number MFCD00078135

  •  PubChem Substance ID 24852036

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Properties

InChI Key   MCBJJSJOIVJYQY-UHFFFAOYSA-J
form   powder
composition   Dye content, 90%
mp   >240 °C (dec.) (lit.)
absorption   λmax 591 nm
  λmax 630 nm (2nd)

Description

Application

New Methylene Blue N zinc chloride double salt has been used as a photosensitizer for the in vitro photodynamic inactivation of plant-pathogenic fungi Colletotrichum acutatum and Colletotrichum gloeosporioides. It has also been used to determine the in vitro susceptibilities of Trichophyton mentagrophytes and Trichophyton rubrum microconidia to photodynamic antimicrobial chemotherapy (PACT).

Packaging

10 g in glass bottle

Biochem/physiol Actions

New Methylene Blue N zinc chloride double salt also known as methylene blue N is an azo dye. It can bind, neutralize and crosslink with RNA. This causes co-precipitation of ribosomal and residual RNA with the mitochondria and ferritin masses. These events make the living, young erythrocytes as microscopically visible, dark-blue clusters and filaments, referred to as reticulum. New Methylene Blue N zinc chloride double salt helps in counting of reticulocytes (erythrocytes containing RNA).

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles
Peer-Reviewed Papers
15

References

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