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227935 Sigma-Aldrich

Azure B

prepared by direct synthesis

Synonym: N,N,N′-Trimethylthionin, Azure I, Methyleneazure

  • CAS Number 531-55-5

  • Empirical Formula (Hill Notation) C15H16ClN3S

  • Molecular Weight 305.83

  •  Colour Index Number 52010

  •  Beilstein/REAXYS Number 3922630

  •  EC Number 208-511-2

  •  MDL number MFCD00011935

  •  PubChem Substance ID 24853664

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Properties

Related Categories A, Cell Biology, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
Quality Level   200
assay   >85.0% (HPLC)
form   powder
application(s)   hematology: suitable
  histology: suitable
mp   205-210 °C (dec.) (lit.)
solubility   water: 1 mg/mL, blue to very deep blue
λmax   648 nm
ε (extinction coefficient)   ≥13000 at 241-247 nm
  ≥36000 at 287-293 nm
  ≥67000 at 638-644 nm
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]
InChI   1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H
InChI key   DNDJEIWCTMMZBX-UHFFFAOYSA-N

Description

Application

Azure B has been used in M′Fadyean stain to visualize Bacillus anthracis capsule.

Packaging

5, 100 g in glass bottle

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films. It is also used for the localization of nucleic acids and lignin.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
SO5550000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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