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230162 Sigma-Aldrich

Sulforhodamine B

Dye content 75 %

Synonym: Acid Red 52, Sulforhodamine B monosodium salt

  • CAS Number 3520-42-1

  • Empirical Formula (Hill Notation) C27H29N2NaO7S2

  • Molecular Weight 580.65

  •  Colour Index Number 45100

  •  Beilstein/REAXYS Number 3886008

  •  EC Number 222-529-8

  •  MDL number MFCD00010180

  •  PubChem Substance ID 24853778

  •  NACRES NA.47

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Properties

form   powder
composition   Dye content, 75%
application(s)   hematology: suitable
  histology: suitable
solubility   methanol: 1 mg/mL
λmax   554 nm
ε (extinction coefficient)   ≥85000 at 554-560 nm in 0.1 M NaOH in methanol at 0.005 g/L
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1
InChI   1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
InChI key   SXQCTESRRZBPHJ-UHFFFAOYSA-M

Description

General description

Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.

Application

Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.

Packaging

1, 5 g in glass bottle

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
RTECS 
BP6750000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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