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302643 Sigma-Aldrich

Trypan Blue

Dye content 60 %

Synonym: Direct blue 14

  • CAS Number 72-57-1

  • Linear Formula C34H24N6O14S4Na4

  • Molecular Weight 960.81

  •  Colour Index Number 23850

  •  Beilstein/REAXYS Number 4360496

  •  EC Number 200-786-7

  •  MDL number MFCD00003969

  •  PubChem Substance ID 24858268

  •  NACRES NA.47

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Properties

Related Categories Cell Biology, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes, T-U-V More...
Quality Level   200
form   powder
composition   Dye content, 60%
application(s)   hematology: suitable
  histology: suitable
mp   >300 °C (lit.)
solubility   H2O: 1 mg/mL
λmax   607 nm
ε (extinction coefficient)   ≥60000 at 603-607 nm in methanol at 0.01  g/L
  60000 at 603-607 nm in methanol
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI   1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI key   GLNADSQYFUSGOU-UHFFFAOYSA-J
Gene Information   human ... PTPN1(5770), PTPRF(5792)

Description

Application

Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Packaging

25, 100 g in glass bottle

Biochem/physiol Actions

Trypan blue is a blue acid dye that contains two azo chromophores. It is a large, hydrophilic, tetrasulfonated anionic dye. It is also used to screen the cytotoxic properties of plant extracts. In addition, trypan blue is used for staining epiretinal membranes during vitrectomy surgery.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
WGK 3
RTECS 
QJ6475000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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