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323829 Sigma-Aldrich

Methyl Green

zinc chloride salt, <0.5% crystal violet, Dye content 80 %

Synonym: Ethyl Green

  • CAS Number 7114-03-6

  • Linear Formula C27H35BrClN3 · ZnCl2

  • Molecular Weight 653.24

  •  Colour Index Number 42590

  •  Beilstein/REAXYS Number 3914106

  •  EC Number 230-415-4

  •  MDL number MFCD00151094

  •  PubChem Substance ID 24859418

  •  NACRES NA.47

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Properties

Related Categories Cell Biology, Hematology and Histology, M, Stains & Dyes, A to Z, Stains and Dyes More...
form   solid
composition   Dye content, 80%
application(s)   hematology: suitable
  histology: suitable
impurities   <0.5% crystal violet
pH range   0.2 - 1.8, yellow to blue
mp   >300 °C (lit.)
solubility   water: 1 mg/mL
λmax   420 nm (2nd)
  635 nm
ε (extinction coefficient)   ≥14000 at 311-317 in water at 0.005 g/L
  ≥50000 at 629-635 in water at 0.005 g/L
  ≥9000 at 420-426 in water at 0.005 g/L
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C
InChI   1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
InChI key   RRKSQWOQZFIQKW-UHFFFAOYSA-J

Description

General description

Methyl Green is a triphenylmethane dye which is mainly used in combination with pyronin. It is suitable for the detection of DNA and RNA.

Packaging

5, 25 g in glass bottle

Application

Used in the Methyl Green-Pyronin methods to demonstrate DNA and RNA in tissue sections.

Other Notes

Zinc chloride salt

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Target organs 
Respiratory system
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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