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40718 Sigma-Aldrich

Diethyl pyrocarbonate

99% (NT)

Synonym: DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride

  • CAS Number 1609-47-8

  • Linear Formula O(COOC2H5)2

  • Molecular Weight 162.14

  •  Beilstein/REAXYS Number 637031

  •  EC Number 216-542-8

  •  MDL number MFCD00009106

  •  PubChem Substance ID 24865430

  •  NACRES NA.22

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Properties

Related Categories Building Blocks, Carbonates, Carbonyl Compounds, Chemical Synthesis, Organic Building Blocks More...
Quality Level   200
assay   99% (NT)
color   APHA: ≤20
refractive index   n20/D 1.398 (lit.)
  n20/D 1.398
bp   93-94 °C/18 mmHg (lit.)
solubility   95% ethanol: 4.5 g/10 mL, clear, colorless
density   1.121 g/mL at 20 °C
  1.101 g/mL at 25 °C (lit.)
shipped in   wet ice
storage temp.   2-8°C
SMILES string   CCOC(=O)OC(=O)OCC
InChI   1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
InChI key   FFYPMLJYZAEMQB-UHFFFAOYSA-N

Description

General description

Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO2 and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.

Application

Diethyl pyrocarbonate may be used in the following studies:
• For the modification of histidyl residues in proteins.
• As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.
• For the modification of linear and supercoiled plasmid DNAs.
• As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.
• For cabethoxylation of histidine residues of actin.

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Packaging

25, 100 mL in poly bottle

Biochem/physiol Actions

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NA 1993 / PGIII
WGK Germany 
3
RTECS 
LQ9350000
Flash Point(F) 
156.2 °F
Flash Point(C) 
69 °C

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Protocols & Articles
Peer-Reviewed Papers
15

References

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