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40718 Aldrich

Diethyl pyrocarbonate

99% (NT)

Synonym: DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride

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Properties

Related Categories Building Blocks, Carbonates, Carbonyl Compounds, Chemical Synthesis, Organic Building Blocks More...
InChI Key   FFYPMLJYZAEMQB-UHFFFAOYSA-N
assay   99% (NT)
color   APHA: ≤20
refractive index   n20/D 1.398(lit.)
  n20/D 1.398
bp   93-94 °C/18 mmHg(lit.)
solubility   95% ethanol: 4.5 g/10 mL, clear, colorless
density   1.121 g/mL at 20 °C
  1.101 g/mL at 25 °C(lit.)
storage temp.   2-8°C

Description

Application

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Diethyl pyrocarbonate may be used in the following studies:
• For the modification of histidyl residues in proteins.
• As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.
• For the modification of linear and supercoiled plasmid DNAs.
• As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.
• For cabethoxylation of histidine residues of actin.

Biochem/physiol Actions

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

Packaging

25, 100 mL in poly bottle

General description

Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO2 and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NA 1993 / PGIII
WGK Germany 
3
RTECS 
LQ9350000
Flash Point(F) 
156.2 °F
Flash Point(C) 
69 °C

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Protocols & Articles

Protocols

The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on

Since its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle at the basis of the method is that RNA is s...
Keywords: AGE, Absorption, Biochemistry, Cancer, Cell disruption, Centrifugation, Chromatography, Cloning, Degradations, Electrophoresis, Eliminations, Gel electrophoresis, Gene expression, Genetics, Homogenization, Molecular biology, Northern blot, Polymerase chain reaction, Precipitation, Separation

Peer-Reviewed Papers
15

References

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