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476870 Sigma-Aldrich

α-Cyano-4-hydroxycinnamic acid


Synonym: α-CCA, α-CHCA, α-Cyano, 4-HCCA, ACCA, alpha-Cyano-4-hydroxycinnamic acid

  • CAS Number 28166-41-8

  • Linear Formula HOC6H4CH=C(CN)CO2H

  • Molecular Weight 189.17

  •  Beilstein/REAXYS Number 3271427

  •  EC Number 248-879-1

  •  MDL number MFCD00004204

  •  PubChem Substance ID 24871310

  •  NACRES NA.22



Related Categories Building Blocks, C10, Carbonyl Compounds, Carboxylic Acids, Chemical Synthesis,
Quality Level   100
assay   99%
mp   245-250 °C (lit.)
storage temp.   2-8°C
SMILES string   OC(=O)\C(=C\c1ccc(O)cc1)C#N
InChI   1S/C10H7NO3/c11-6-8(10(13)14)5-7-1-3-9(12)4-2-7/h1-5,12H,(H,13,14)/b8-5+


General description

α-Cyano-4-hydroxycinnamic acid is the commonly used matrix for analyzing peptides by matrix-assisted laser desorption/ionization mass spectrometry. It inhibits the monophenolase activity and diphenolase activity of mushroom tyrosinase.


α-Cyano-4-hydroxycinnamic acid (CHC) was used in encapsulation of CHC into NaY zeolite. It was used as matrix to investigate the activity of the paclitaxel derivatives using several well-established in vitro angiogenesis assays.


2, 10 g in glass bottle

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Target organs 
Respiratory system
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What can be used for solublization ofα-Cyano-4-hydroxycinnamic acid?
α-Cyano-4-hydroxycinnamic acid (α-CCA, α-CHCA, α-Cyano, 4-HCCA, ACCA) is soluble in methanol (up to 50 mg/ml). It is also soluble at 10 mg/ml using 50% acetonitrile in 0.05% TFA for MALDI-MS. The acetonitrile concentration can be adjusted for individual preferences.For biological applications, it has been solubilized at 100 mM in DMSO or 50 mM in ethanol.
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