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765392 Sigma-Aldrich

Gold nanoparticles

40 nm diameter, amine functionalized, PEG 5000 coated, OD 50, dispersion in H2O

Synonym: Au NP NH2, Gold Colloid, Gold nanoparticles NH2 functionalized

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Description

Packaging

1 mL in poly bottle

Application

Functionalized particles have been extensively used to study cellular uptake of nanoparticles and targeted drug delivery.

Several other specific applications have been listed below:
• Since they adhere to cell membranes, AuNPs are used in cellular and intracellular targeting in targeted drug delivery applications and can also be used in biodistribution studies
• AuNPs may also be used in photothermal therapy and radiotherapy.
• AuNPs ranging from 10 to 250 nm, were studied to have been absorbed primarily by liver and spleen, when injected intravenously in rats.
• They may also be used in carrying siRNA which acts against human prostate carcinoma cells by inhibiting a specific cancer gene.

Features and Benefits

• A negatively charged siRNA-PEG complex attached to positively charged GNPs is easily cleavable in reductive cytosolic environment thus enabling the release of siRNA into cytosol.
• The PEG coating decreases the cytotoxicity and increases effciciency of GNPs. PEG increases the stability of the nanoparticles and prevents agglomeration.

General description

Gold nanoparticles (AuNPs) ranging from 2-200nm in diameter find applications in diverse fields. They show excellent biocompatibility and attractive physicochemical properties. AuNPs, as aggregates, is shown to be highly sensitive colloidal biosensors. Poly (ethylene) glycol (PEG) surface coating on gold nanoparticles helps in the reduction of protein adsorption, reduces nonspecific interactions with cells and greatly improves the pharmokinetics of these nanoparticles and disables aggregation of the nanoparticles. PEG layer density depends inversely on the AuNP curvature. Denser coating was possible on smaller AuNPs. AuNPs coated with PEG 5000 exhibited highest colloidal stability. Amine functionalized GNPs which has Poly Ethylene Glycol (PEG) as the ligand or carrier molecule forms a conjugate with siRNA and is extremely useful in RNAi technology. Since it adheres to cell membranes, it may find use in cellular and intracellular targeting in targeted drug delivery applications and also used in biodistribution studies.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Webinar - Hydrogels
Protocols & Articles

Articles

Magnetic Nanoparticles for Cancer Theranostics

Saumya Nigam,1 Lina Pradhan,2 and D. Bahadur*3 1IITB-Monash Research Academy, IIT Bombay, Powai, Mumbai, India 2Centre for Research in Nanotechnology & Science IIT Bombay, Powai, Mumbai, India 3Depar...
Keywords: Apoptosis, Bacterial conjugations, Cancer, Diagnostic, Magnetic resonance imaging, Materials Science, Nanomaterials, Nanotechnology, Reductions

Micro- and Nanofabricated Biomaterials for the Study of Cells and Microtissues

Phin Peng Lee, Karen E. Samy, Miquella G. Chavez, Alec Cerchiari, Tejal A. Desai* Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA 9415...
Keywords: Absorption, Adhesion, Amplification, Apoptosis, Biomaterials, Cancer, Cell culture, Cell proliferation, Diffusion, Immobilization, Indicators, Infrared spectroscopy, Ligands, Magnetic resonance imaging, Materials Science, Microscopy, Molecular biology, Nanotechnology, Semiconductor, Solvents

Peer-Reviewed Papers
15

References

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