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861030 Sigma-Aldrich

Auramine O

Dye content 85 %, certified by the Biological Stain Commission

Synonym: 4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum

  • CAS Number 2465-27-2

  • Empirical Formula (Hill Notation) C17H21N3 · HCl

  • Molecular Weight 303.83

  •  Colour Index Number 41000

  •  Beilstein/REAXYS Number 4030061

  •  EC Number 219-567-2

  •  MDL number MFCD00012484

  •  PubChem Substance ID 24888527

  •  NACRES NA.47

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains, Other Fluorescent Labels
Quality Level   200
grade   certified by the Biological Stain Commission
composition   Dye content, 85%
mp   >250 °C (dec.) (lit.)
λmax   370 nm
  434 nm (2nd)
SMILES string   Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C
InChI   1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
InChI key   KSCQDDRPFHTIRL-UHFFFAOYSA-N

Description

Application

Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta.

Packaging

25 g in glass bottle

Biochem/physiol Actions

Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Suitability

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
UN 2811 6.1 / PGIII
WGK Germany 
WGK 3
RTECS 
BY3675000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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