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861049 Sigma-Aldrich

Azure A chloride

certified by the Biological Stain Commission, Dye content 70 %

  • CAS Number 531-53-3

  • Empirical Formula (Hill Notation) C14H14ClN3S

  • Molecular Weight 291.80

  •  Colour Index Number 52005

  •  Beilstein Registry Number 3922287

  •  EC Number 208-510-7

  •  MDL number MFCD00012112

  •  PubChem Substance ID 329769437

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Properties

grade   certified by the Biological Stain Commission
InChI Key   NALREUIWICQLPS-UHFFFAOYSA-N
form   powder
composition   Dye content, 70%
mp   290 °C (dec.) (lit.)
solubility   H2O: 1 mg/mL
absorption   extinction-1/242-246 nm, H2O ≥11000
  extinction/287-291 nm, H2O ≥26000
  extinction/620-634 μm, H2O ≥34000
  λmax 633 nm

Description

Packaging

5, 25 g in glass bottle

Application

Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles.

Biochem/physiol Actions

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.

Suitability

Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
SP5660000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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