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861049 Sigma-Aldrich

Azure A chloride

certified by the Biological Stain Commission, Dye content 70 %

  • CAS Number 531-53-3

  • Empirical Formula (Hill Notation) C14H14ClN3S

  • Molecular Weight 291.80

  •  Colour Index Number 52005

  •  Beilstein/REAXYS Number 3922287

  •  EC Number 208-510-7

  •  MDL number MFCD00012112

  •  PubChem Substance ID 329769437

  •  NACRES NA.47

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Properties

Quality Level   200
grade   certified by the Biological Stain Commission
form   powder
composition   Dye content, 70%
application(s)   hematology: suitable
  histology: suitable
mp   290 °C (dec.) (lit.)
solubility   H2O: 1 mg/mL
λmax   633 nm
ε (extinction coefficient)   ≥11000 at 242-246 nm in H2O at -1
  ≥26000 at 287-291 nm in H2O
  ≥34000 at 620-634 μm in H2O
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]
InChI   1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
InChI key   NALREUIWICQLPS-UHFFFAOYSA-N

Description

Application

Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles.

Packaging

5, 25 g in glass bottle

Biochem/physiol Actions

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.

Suitability

Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
SP5660000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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