• USA Home
  • 913839 - 2-Methylnicotinic acid imidazolide

EMAIL THIS PAGE TO A FRIEND
913839 Sigma-Aldrich

2-Methylnicotinic acid imidazolide

New

Synonym: (1H-imidazol-1-yl)(2-methylpyridin-3-yl)methanone, 1H-Imidazol-1-yl(2-methyl-3-pyridinyl)methanone, 3-(1H-imidazole-1-carbonyl)-2-methylpyridine, NAI, RNA SHAPE probe, RNA structure probe, mNAI

Purchase

Description

Application

2-Methylnicotinic acid imidazolide (NAI or mNAI) is an RNA SHAPE probe for live-cell RNA structure profiling. SHAPE -- or selective 2′-hydroxyl acylation analyzed by primer extension -- uses small, electrophilic chemical probes to react with the 2′-hydroxyl group and provides insight to RNA structure and its dynamics in varied cells states or in cells with gene knockouts. It has also been extended to the SHALiPE approach, selective 2′-hydroxyl acylation with lithium ion-based primer extension.

Other Notes

Conditional control of RNA-guided nucleic acid cleavage and gene editing

Guidelines for SHAPE Reagent Choice and Detection Strategy for RNA Structure Probing Studies

SnapShot: RNA Structure Probing Technologies

Structural Analysis using SHALiPE to Reveal RNA G-Quadruplex Formation in Human Precursor MicroRNA

Determination of in vivo RNA structure in low-abundance transcripts

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles
Related Products

related product

Product #

Description

Add to Cart

03-310 In Vivo SHAPE Reagent for Live Cell RNA Structure Analysis, In Vivo SHAPE Reagent contains 2-methylnicotinic acid imidazolide (NAI). This reagent has very low toxicity and permits the analysis of RNA structure in living cells using Selective 2’-Hydroxyl Acylation and Primer Extension (SHAPE).
908401 1-Methyl-7-nitroisatoic anhydride
914487 2-(Diphenylphosphanyl)benzamide, ≥95%
914983 ThT-NE, ≥95%
115959 Benzoyl cyanide, 98%
320293 Dimethyl sulfate, ≥99%

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?