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L020000-HH Aldrich

TCA Deblock

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Properties

Related Categories ABI 3900, ABI 39X, Chemical Synthesis, Deblock, Expedite,
type   for DNA synthesis
product line   Proligo Reagents
assay   28-32 g/L (TCA content, titration)
packaging   pkg of 4 × 2.5 L (L020250-04)
  pkg of 1 × 25 L (L022500-01)
  pkg of 4 × 4 L (L020400-04)
impurities   ≤200 ppm water content (Karl Fischer)
color   clear

Description

Other Notes

Deblock solution containing trichloroacetic acid (TCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Preparation Note

Trichloroacetic acid / Dichloromethane 3/100 (v/v)

Safety & Documentation

Safety Information

Signal word 
Danger
RIDADR 
UN2922 - class 8 - PG 3 - EHS - acidic - Corrosive liquids, toxic, n.o.s., HI: all
WGK Germany 
2
Flash Point(F) 
212 °F
Flash Point(C) 
100 °C

Documents

Certificate of Analysis

Protocols & Articles
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