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L030000 Sigma-Aldrich

ETT Activator

0.25 M

Synonym: 5-(Ethylthio)-1H-tetrazole, 5-(Ethylthio)tetrazole solution

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Properties

Related Categories Activator, Chemical Synthesis, ETT Activator, Phosphoramidites and Reagents for Oligonucleotide Synthesis (Proligo Reagents)
type   for DNA synthesis
product line   Proligo Reagents
assay   32-35 g/L (ETT content, titration)
storage condition   dry at room temperature
impurities   ≤30 ppm water content (Karl Fischer)
color   clear
SMILES string   CCSc1nnn[nH]1
InChI   1S/C3H6N4S/c1-2-8-3-4-6-7-5-3/h2H2,1H3,(H,4,5,6,7)
InChI key   GONFBOIJNUKKST-UHFFFAOYSA-N

Description

Packaging

4×2, 4×4 L in glass bottle

6×450 mL in glass bottle

Preparation Note

Acetonitrile / 5-(Ethylthio)-1H-Tetrazole 100 / 3 (v/w)

Other Notes

Activator solution, containing 5-(Ethylthio)-1H- tetrazole (ETT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.

Safety & Documentation

Safety Information

RIDADR 
UN 1993BE 3 / PGII

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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