EMAIL THIS PAGE TO A FRIEND
L030080 Sigma-Aldrich

ETT Activator

0.25 M, configured for PerkinElmer, configured for Polygen, configured for MerMade, configured for ABI 3900

Synonym: 5-(Ethylthio)-1H-tetrazole, 5-(Ethylthio)tetrazole solution

Purchase

Properties

Related Categories Activator, Chemical Synthesis, ETT Activator, Expedite, Liquid Reagents,
type   for DNA synthesis
product line   Proligo Reagents
assay   32-35 g/L (ETT content, titration)
storage condition   dry at room temperature
impurities   ≤30 ppm water content (Karl Fischer)
color   clear
compatibility   configured for ABI 3900
  configured for MerMade
  configured for PerkinElmer
  configured for Polygen
SMILES string   CCSc1nnn[nH]1
InChI   1S/C3H6N4S/c1-2-8-3-4-6-7-5-3/h2H2,1H3,(H,4,5,6,7)
InChI key   GONFBOIJNUKKST-UHFFFAOYSA-N

Description

Preparation Note

Acetonitrile / 5-(Ethylthio)-1H-Tetrazole 100 / 3 (v/w)

Other Notes

Activator solution, containing 5-(Ethylthio)-1H- tetrazole (ETT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 1993BE 3 / PGII
WGK Germany 
3
Flash Point(F) 
35.6 °F
Flash Point(C) 
2 °C

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?