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L033000 Sigma-Aldrich

BTT Activator

0.3 M

Synonym: 5-(Benzylthio)-1H-tetrazole solution

  • Empirical Formula (Hill Notation) C8H8N4S

  • Molecular Weight 192.24

  •  MDL number MFCD00068731

  •  PubChem Substance ID 329817353

  •  NACRES NA.21

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Properties

Related Categories Activator, BTT Activator, Chemical Synthesis, ETT Activator, Phosphoramidites and Reagents for Oligonucleotide Synthesis (Proligo Reagents) More...
Quality Level   200
type   for DNA synthesis
product line   Proligo Reagents
assay   51.9-63.4 g/L (BTT content, titration)
storage condition   dry at room temperature
color   clear
SMILES string   C(Sc1nnn[nH]1)c2ccccc2
InChI   1S/C8H8N4S/c1-2-4-7(5-3-1)6-13-8-9-11-12-10-8/h1-5H,6H2,(H,9,10,11,12)
InChI key   GXGKKIPUFAHZIZ-UHFFFAOYSA-N

Description

Preparation Note

Acetonitrile / 5-(Benzylthio)-1H-Tetrazole 100 / 6 (v/w)

Other Notes

Activator solution, containing 5-(Benzylthio)-1H-Tetrazole (BTT or BMT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 1648 3 / PGII
WGK Germany 
3
Flash Point(F) 
41 °F
Flash Point(C) 
5 °C

Documents

Certificate of Analysis (COA)

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