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L040150 Sigma-Aldrich

Cap 1

Synonym: Cap A

  •  NACRES NA.21

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Properties

Related Categories Cap A, Chemical Synthesis, Phosphoramidites and Reagents for Oligonucleotide Synthesis (Proligo Reagents) More...
type   for DNA synthesis
product line   Proligo Reagents
assay   conforms (Formulation, GC)
storage condition   dry at room temperature
impurities   ≤200 ppm water content (Karl Fischer)
color   clear

Description

Preparation Note

Acetonitrile / Lutidine / N-Methylimidazole 8/1/1 (v/v/v)

Other Notes

Cap 1 solution, containing n-methylimidazole, lutidine and acetonitrile (ACN), is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2924 8(3) / PGII
WGK Germany 
3
Flash Point(F) 
35.6 °F
Flash Point(C) 
2 °C

Documents

Certificate of Analysis (COA)

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