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M6250 Aldrich

2-Mercaptoethanol

≥99.0%

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Widely used for retarding oxidation of biological compounds in solution.

Packaging

1, 2.5 L in glass bottle

10, 100, 250, 500 mL in glass bottle

General description

2-mercaptoethanol is a thiol compound, commonly used as a reducing agent in organic reactions.

Price and Availability


LC-MS Grade Solvents and Reagents

Amplified Detection
Safety & Documentation

Safety Information

Signal word 
Danger
Target organs 
Heart, Liver
RIDADR 
UN 2966 6.1 / PGII
WGK Germany 
3
RTECS 
KL5600000
Flash Point(F) 
165.2 °F
Flash Point(C) 
74 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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