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M6250 Aldrich

2-Mercaptoethanol

≥99.0%

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

  • CAS Number 60-24-2

  • Linear Formula HSCH2CH2OH

  • Molecular Weight 78.13

  •  Beilstein/REAXYS Number 773648

  •  EC Number 200-464-6

  •  MDL number MFCD00004890

  •  PubChem Substance ID 57654402

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Description

General description

2-mercaptoethanol is a thiol compound, commonly used as a reducing agent in organic reactions.

Packaging

1, 2.5 L in glass bottle

10, 100, 250, 500 mL in glass bottle

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Widely used for retarding oxidation of biological compounds in solution.

Safety & Documentation

Safety Information

Signal word 
Danger
RIDADR 
UN 2966 6.1 / PGII
WGK Germany 
3
RTECS 
KL5600000
Flash Point(F) 
165.2 °F
Flash Point(C) 
74 °C

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Protocols

Protocols for the Fmoc SPPS of Cysteine-containing Peptides

Peptides containing Cys can exist in either reduced (sulfhydryl) or oxidized inter/intra chain disulfide bonded forms. The synthesis of peptides containing Cys, therefore, presents special challenges...
Keywords: Alkylations, Asymmetric synthesis, Buffers, Centrifugation, Coupling reactions, Cyclizations, Eliminations, Evaporation, Filtration, High performance liquid chromatography, Hormones, Iodinations, Oxidations, Peptide synthesis, Purification, Racemizations, Rearrangements, Reductions, Solid phase peptide synthesis, Solvents, Thiolysis

Peer-Reviewed Papers
15

References

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