M6250 Sigma-Aldrich



Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

  • CAS Number 60-24-2

  • Linear Formula HSCH2CH2OH

  • Molecular Weight 78.13

  •  Beilstein Registry Number 773648

  •  EC Number 200-464-6

  •  MDL number MFCD00004890

  •  PubChem Substance ID 57654402




BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Widely used for retarding oxidation of biological compounds in solution.


1, 2.5 L in glass bottle

10, 100, 250, 500 mL in glass bottle

General description

2-mercaptoethanol is a thiol compound, commonly used as a reducing agent in organic reactions.

Safety & Documentation

Safety Information

Signal word 
UN 2966 6.1 / PGII
WGK Germany 
Flash Point(F) 
165.2 °F
Flash Point(C) 
74 °C

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Protocols & Articles


Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis

The transient gene expression system using Arabidopsis mesophyll protoplasts has proven an important and versatile tool for conducting cell-based experiments using molecular, cellular, biochemical, g...
Keywords: Gene expression, Genetic, Genetics, Molecular biology, Transfection

Blue native PAGE

a Molekulare Bioenergetik, Zentrum der Biologischen Chemie, Universitätsklinikum Frankfurt, Theodor-Stern-Kai 7, Haus 26
Keywords: Crystallization, Diffusion, Electroblotting, Isoelectric focusing, Microscopy, PAGE, Scanning electron microscopy, Separation

Protocols for the Fmoc SPPS of Cysteine-containing Peptides

Peptides containing Cys can exist in either reduced (sulfhydryl) or oxidized inter/intra chain disulfide bonded forms. The synthesis of peptides containing Cys, therefore, presents special challenges...
Keywords: Alkylations, Asymmetric synthesis, Buffers, Centrifugation, Coupling reactions, Cyclizations, Eliminations, Evaporation, Filtration, High performance liquid chromatography, Hormones, Iodinations, Oxidations, Peptide synthesis, Purification, Racemizations, Rearrangements, Reductions, Solid phase peptide synthesis, Solvents, Thiolysis

The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on

Since its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle at the basis of the method is that RNA is s...
Keywords: AGE, Absorption, Biochemistry, Cancer, Cell disruption, Centrifugation, Chromatography, Cloning, Degradations, Electrophoresis, Eliminations, Gel electrophoresis, Gene expression, Genetics, Homogenization, Molecular biology, Northern blot, Polymerase chain reaction, Precipitation, Separation


a Molekulare Bioenergetik, Zentrum der Biologischen Chemie, Universitätsklinikum Frankfurt, Theodor-Stern-Kai 7, Haus 26
Keywords: Buffers, Chromatography, Crystallization, Degradations, Detergents, Edman degradation, Electroblotting, Electrophoresis, Gel electrophoresis, PAGE, Phosphorylations, Polymerization reactions, Proteomics, Purification, Respiratory, SDS PAGE, Sample preparations, Separation, Sequencing, Solvents, Western blot

Peer-Reviewed Papers


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