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QBD10379 Sigma-Aldrich

SPDP-dPEG®24-NHS ester

  • Empirical Formula (Hill Notation) C63H113N3O29S2

  • Molecular Weight 1440.70

  •  MDL number MFCD13185007

  •  PubChem Substance ID 329823924

  •  NACRES NA.22



Related Categories Amine to Sulfhydryl, Chemical Biology, Chemical Synthesis, Crosslinkers, Heterobifunctional,
assay   >90%
form   solid or viscous liquid
reaction suitability   reagent type: cross-linking reagent
reactivity: amine reactive
  reagent type: cross-linking reagent
reactivity: thiol reactive
polymer architecture   shape : linear
functionality : heterobifunctional
shipped in   ambient
storage temp.   −20°C


Features and Benefits

SPDP-dPEG®24-NHS ester has an amine-reactive N-hydroxysuccinimide (NHS) ester at one end and a sulfhydryl-reactive 2-pyridyldithiol group at the opposite end. This has a single molecular weight PEG spacer arm, which confers greater water solubility to the crosslinker and linked proteins compared to crosslinkers having only hydrocarbon spacers. Protein aggregation and precipitation also are greatly reduced with SPDP-dPEG crosslinkers compared to crosslinkers that have only hydrocarbon spacers. dPEG spacer of specific length (97.3 Å) provides precise distance control between the SPDP moiety and the target molecule. Pyridyldithiol reagents produce disulfide-containing linkages that can be cleaved with reducing agents such as dithiothreitol (DTT). Crosslinking experiments with SPDP reagents are not limited to those involving proteins. Any of a variety of molecules with primary amines and sulfhydryl groups can be modified or crosslinked using SPDP reagents.

Legal Information

Products Protected under U.S. Patents # 7,888,536 B2

dPEG is a registered trademark of Quanta BioDesign

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


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