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330021S Avanti

WR304 mAb

WR304 Mouse Monoclonal Antibody (IgM), Anti-(PIP/PIP2), 1X TBS (Tris Buffered Saline) with 1mM Na2 EDTA

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Properties

Related Categories Avanti Lipidomics Products, Avanti Lipidomics Toolbox, Avanti Polar Lipids Products, Biochemicals and Reagents, Lipids More...
antibody product type   primary antibodies
assay   >95%
packaging   pkg of 1 × 0.1 mL (polypropylene tube with screw cap (330021S-100ug))
mfr. no.   Avanti Polar Lipids, 330021S
concentration   1 mg/mL (330021S-100ug)
shipped in   dry ice
storage temp.   −70°C

Description

General description

WR304 is an antibody that binds specifically to the lipids PIP and PIP2. WR304 also neutralizes infectious HIV-1 virus and can be used to probe the presence of PIP and PIP2 in membranes in a variety of applications including inositol phosphate-dependent cell signaling pathways. This antibody was developed by the Walter Reed Army Institute of Research (WRAIR) using a targeted adjuvant protocol with liposomes containing Avanti’s Lipid A.
Formulation: WR304 is provided as a 0.2μm sterile-filtered solution in Tris buffered saline (TBS). WR304 binding to porcine brain PI(4)P is inhibited by high concentration of Ca2+ (10 mM)iv and addition of 1 mM EDTA stimulates this binding activity. PBS and other phosphate-containing buffers should not be used as they inhibit binding to the phospholipid head groupiii.

Specificity

WR304 recognizes PIP and PIP2, and neutralizes HIV-1 virus.

Immunogen

Antigens: Brain PI(4)P

Application

ELISA, IHC, Dot Blot, Flow cytometry and HIV-1 infectivity.

The WR304 antibody has been used for the quantitation of PIP/PIP2 in direct ELISA and Dot Blot analysis. The WR304 antibody specifically binds to PIP/PIP2 and inhibits the infectivity of HIV-1 to peripheral blood mononuclear cells (PBMC).

Packaging

0.5 mL Polypropylene Tube with Screw Cap (330021S-100ug)

Storage and Stability

Store undiluted at either -20°C or -80°C.

Other Notes

Hazardous/Non-hazardous Components: This product contains no substances that, at their given concentration, are known to be hazardous to health. Therefore, there is no MSDS for this product.

Ig Class: Mouse IgM (kappa).
Antibody Source: Monoclonal antibody from BALB/c-derived hybridoma WR304.
Production: In vitro cell culture. Purification: Ultra filtration through 100 KDa cut-off filters.
Purification: Ultra filtration through 100 KDa cut-off filters.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Storage & Handling of Lipids - Avanti® Polar Lipids

The following statements pertain specifically to Avanti’s lipid products for research and should not necessarily be applied to cGMP lipids intended for pharmaceutical or diagnostic applications.
Keywords: Diagnostic, Oxidations, Pharmaceutical, Solvents

Protocols

General Lipid/BSA Solubilization Protocol For Cell Delivery

Water Solutions: Solubilizing some lipids is difficult using organic solvents. Typically, lipids are suspended in methanol to aliquot, the methanol is then dried and the residue dispersed using fatty...
Keywords: Evaporation, Solvents

Peer-Reviewed Papers
15

References

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