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360205P Avanti

Huzzah® NBD-So

Huzzah® NBD-So, Human Serum Albumin / NBD Sphingosine Complex, powder

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Properties

Related Categories Avanti Lipidomics Products, Avanti Lipidomics Toolbox, Avanti Polar Lipids Products, Biochemicals and Reagents, Lipids More...
assay   >99% (MS)
form   powder
mfr. no.   Avanti Polar Lipids, 360205P
packaging   1 ea of 1 × (with stopper and crimp cap)
shipped in   dry ice
storage temp.   −20°C

Description

Packaging

10 mL Clear Glass Vial with Stopper and Crimp Cap (360205P-1EA)

General description

Avanti′s new HSA lipid delivery system, Huzzah® improves the cellular delivery of NBD-sphingosine through its conjugation with human serum albumin (HSA), a physiologically relevant carrier protein. This conjugate system eliminates the need for organic solvents, which are well-known to adversely affect viability and the phenotypic characteristics of cells. The Huzzah® lipid delivery system is supplied as a lyophilized conjugate which can be directly and easily reconstituted in water or a buffer of choice and is ideal for studying the effects of poorly soluble bioactive molecules.Solubility: Reconstitute lyophilized conjugate in 5 mL of water or other aqueous buffer. This produces a stock solution of 120 - 200 μM NBD-So*. A short sonication may be necessary to ensure complete dissolution. If necessary, filter sterilize the reconstituted conjugate prior to use in cell culture applications. *Exact concentration will vary from batch to batch. Refer to label for exact product concentration upon reconstitution as determined by mass spectrometric analysis. Molar Ratio: NBD-So:albumin (1:1) Albumin Biological Characterization: microbial free; endotoxin < 1 IU/mL Albumin Lipid Characterization: ~0.07g/L phospholipids; ~0.02 g/L sphingolipids; ~0.1 g/L fatty acids; and triglycerides and cholesterols below the limit of quantitation of 0.1 g/L and 0.05 g/L

Legal Information

Huzzah is a registered trademark of Avanti Polar Lipids, Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Webinar - Hydrogels
Protocols & Articles

Articles

Storage & Handling of Lipids - Avanti® Polar Lipids

The following statements pertain specifically to Avanti’s lipid products for research and should not necessarily be applied to cGMP lipids intended for pharmaceutical or diagnostic applications.
Keywords: Diagnostic, Oxidations, Pharmaceutical, Solvents

Protocols

General Lipid/BSA Solubilization Protocol For Cell Delivery

Water Solutions: Solubilizing some lipids is difficult using organic solvents. Typically, lipids are suspended in methanol to aliquot, the methanol is then dried and the residue dispersed using fatty...
Keywords: Evaporation, Solvents

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