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850306C Avanti

07:0 PC (DHPC)

1,2-diheptanoyl-sn-glycero-3-phosphocholine, chloroform

Synonym: DHPC; PC(7:0/7:0)

  • CAS Number 39036-04-9

  • Empirical Formula (Hill Notation) C22H44NO8P

  • Molecular Weight 481.56

  •  NACRES NA.25

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Properties

Related Categories Avanti PC & LPC, Avanti Phospholipids, Avanti Polar Lipids Products, Biochemicals and Reagents, Lipids More...
assay   >99% (TLC)
packaging   pkg of 1 × 20 mL (850306C-500mg)
  pkg of 2 × 4 mL (850306C-200mg)
mfr. no.   Avanti Polar Lipids, 850306C
concentration   25 mg/mL (850306C-200mg)
  25 mg/mL (850306C-500mg)
shipped in   dry ice
storage temp.   −20°C
InChI   1S/C22H44NO8P/c1-6-8-10-12-14-21(24)28-18-20(31-22(25)15-13-11-9-7-2)19-30-32(26,27)29-17-16-23(3,4)5/h20H,6-19H2,1-5H3/t20-/m1/s1
InChI key   RBFSPQDASPEAID-HXUWFJFHSA-N

Description

General description

07:0 PC (DHPC)/1,2-diheptanoyl-sn-glycero-3-phosphocholine, is a phospholipidwhich functions as a detergent. Phosphatidylcholine (PC) is a strong bilayer-forming lipid. It the most common phospholipid in mammalian membranes. It is also an important component of the mucosal layer of the colon.

The list of Phosphatidylcholine products offered by Avanti is designed to provide compounds having a variety of physical properties. Products available include short chain (C3-C8 are water soluble and hygroscopic), saturated, multi-unsaturated and mixed acid PC′s. All of the products are purified by HPLC, and special precautions are taken to protect the products for oxidization and hydrolysis. Preserve the Activity of Solubilized Membrane ProteinsProtein Preservation DHPC preserves the native conformation and therefore the activity of the solubilized proteins. The three dimensional structure and activity of proteins are retained not only at the 10-15mM DHPC concentrations at which maximum solubilization usually occurs, but also over a large range of DHPC concentrations (up to 40mM). Not only is the three dimensional structure and hence the activity of most protein retained in excess DHPC, but the proteins also appear to be stable in DHPC. Such a result suggests that the direct interaction of DHPC with integral membrane proteins is weak. If the interaction of DHPC with membrane proteins is indeed weak, it follows that intrinsic membrane lipids will remain associated with the membrane proteins. Solubilization Mechanism DHPC is thought to exert a wedge-like effect on the neighboring lipids, mainly due to its bulky polar group and short hydrocarbon chains. This produces membrane destabilization at relatively low DHPC concentrations. All membranes investigated are solubilized at an identical DHPC / lipid ratio. This finding is taken as evidence that DHPC primarily interacts with the lipid bilayer and not with the membrane proteins. The principle underlying the preservation of the native protein structure is the inability of DHPC to displace intrinsic membrane lipids from integral membrane proteins.Advantages of DHPC: DHPC preserves the activity of solubilized membrane proteins. DHPC is not readily oxidized, and is stable over a wide pH range (4-10). DHPC forms micelles rather than bilayers when dispersed in water. (CMC = 1.4mM). DHPC shows a broad size distribution depending on the NaCl concentration of suspension. DHPC does not interfere with spectrophotometric measurements. DHPC is an ultra pure compound.

Application

07:0 PC (DHPC) is suitable for use:
• in the preparation of vesicles
• to test the stability of purified cytochrome b558
• in the formation of lipid bicelles
• as an additive added to the supernatant

Packaging

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (850306C-500mg)

5 mL Clear Glass Sealed Ampule (850306C-200mg)

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Target organs 
Central nervous system
RIDADR 
UN 1888 6.1 / PGIII
WGK Germany 
WGK 3
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Critical Micelle Concentrations (CMCs) - Avanti® Polar Lipids

The critical micelle concentration (CMC) can be approximately defined as the lipid monomer concentration at which appreciable amounts (>5% of total) of micellar aggregates first begin to appear in th...
Keywords: Biochemistry

Storage & Handling of Lipids - Avanti® Polar Lipids

The following statements pertain specifically to Avanti’s lipid products for research and should not necessarily be applied to cGMP lipids intended for pharmaceutical or diagnostic applications.
Keywords: Diagnostic, Oxidations, Pharmaceutical, Solvents

Protocols

Determination of Phospholipid Oxidation by UV/VIS Spectroscopy - Avanti® Polar Lipids

The percent oxidation of a phospholipid can be determined by using Beer’s Law to calculate the peroxidized phospholipid’s concentration from its absorbance at 234 nm.
Keywords: Extinction coefficient, Oxidations

General Lipid/BSA Solubilization Protocol For Cell Delivery

Water Solutions: Solubilizing some lipids is difficult using organic solvents. Typically, lipids are suspended in methanol to aliquot, the methanol is then dried and the residue dispersed using fatty...
Keywords: Evaporation, Solvents

Peer-Reviewed Papers
15

References

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