• USA Home
  • H-086 - 25-Hydroxyvitamin D3 solution

EMAIL THIS PAGE TO A FRIEND
H-086 Supelco

25-Hydroxyvitamin D3 solution

5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

Purchase

Properties

Related Categories Additional Standards, Analytical Standards, Analytical/Chromatography, Biomarkers and Metabolomics Standards, Certified Reference Materials (CRMs),
grade   certified reference material
InChI Key   JWUBBDSIWDLEOM-DTOXIADCSA-N
feature   Snap-N-Spike®/Snap-N-Shoot®
packaging   ampule of 1 mL
mfr. no.   Cerilliant®
concentration   5 μg/mL in ethanol
format   single component solution
storage temp.   −70°C

Description

General description

A Certified Spiking Solution® of 25-Hydroxyvitamin D3, a prohormone whose blood serum levels are used to determine Vitamin D nutritional status. This solution standard is suitable for critical quantitative applications in research and clinical environments including: for use in preparation of controls and calibrators for LC/UV and LC-MS/MS assays; as system tuning/suitability standards; linearity verification; internal proficiency testing; and for OEM use in preparation of reagents, development of assays and instrument IQ/OQ. The stable, quantitative solution format provides significant benefits to the user including material savings, increased efficiency of labor, and convenience of use. The shelf stable Snap-N-Spike® format allows laboratories to eliminate painstaking weighing operations and provides the ability to spike a known concentration of analyte into their matrix of choice just before use.

Legal Information

CERILLIANT is a registered trademark of Cerilliant Corporation

CERTIFIED SPIKING SOLUTION is a registered trademark of Cerilliant Corporation

Snap-N-Shoot is a registered trademark of Cerilliant Corporation

Snap-N-Spike is a registered trademark of Cerilliant Corporation

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN1170 - class 3 - PG 2 - Ethanol, solution
WGK Germany 
1
Flash Point(F) 
57.2 °F
Flash Point(C) 
14 °C

Documents

Certificate of Analysis

Please Enter a Lot Number
Protocols & Articles

Articles

Fast and Accurate Analysis of Vitamin D Metabolites Using Novel Chromatographic Selectivity and Sample Preparation

Rat serum was spiked at 300 ng/mL with 25-hydroxyvitamin D2, 25- hydroxyvitamin D3 and epi-25-hydroxyvitamin D3. Protein precipitation was performed offline by adding 100 μL of spiked serum into a 50...
Craig Aurand1, David Bell1, Anders Fridstrom2, and Klaus Buckendahl2
1Supelco, Div. of Sigma-Aldrich, Bellefonte, PA 16823 USA 2Sigma-Aldrich Chemie GmbH, 82024 Taufkirchen, Germany
Keywords: Absorption, Detection methods, High performance liquid chromatography, Immunoassay, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Precipitation, Sample preparations, Separation, Solvents, Vitamins

HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 20 °C

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 20 °C
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 30 °C

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 30 °C
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 40 °C

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 40 °C
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

HPLC Analysis of 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 on Ascentis® Express F5
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

HPLC Analysis of 25-Hydroxyvitamin D3 and epi-25-Hydroxyvitamin D3 on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 25-Hydroxyvitamin D3 and epi-25-Hydroxyvitamin D3 on Ascentis® Express F5
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

Hormone Standards

Over the past 17 years, hormone testing has increased significantly in the clinical laboratory setting as advances in the specificity and selectivity of LC-MS/MS technology have enabled hormones to s...
Keywords: Clinical, Clinical Chemistry, Diagnostic, Diseases, Endocrinology, High performance liquid chromatography, Hormones, Ion-exchange chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites

Improvement in LC/MS Analysis of Vitamin D Metabolites in Serum by Leveraging Column Selectivity and Effective Sample Prep

Clinical interest in vitamin D stems from its implications in an ever-widening range of human health conditions.1 This heightened interest has spawned the need for analytical strategies to assess an ...
Craig Aurand
Reporter US Vol 31.1
Keywords: Clinical, High performance liquid chromatography, Immunoassay, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Metabolites, Precipitation, Sample preparations, Separation, Solid phase extractions, Solvents, Vitamins

LC/MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 on Ascentis® Express F5
Keywords: Chromatography, Clinical, High performance liquid chromatography, Indicators, Liquid chromatography mass spectrometry, Mass spectrometry, Solvents, Vitamins

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 (Monitored Ions)

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 (Monitored Ions)
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Solvents, Vitamins

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Solvents, Vitamins

LC/MS/MS of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3, and 3-epi-25-Hydroxyvitamin D3 in Serum or Plasma on Ascentis® Express F5 after SPE with HybridSPE®-Phospholipid, Minimization of Matrix Effects

From our library of Articles, Sigma-Aldrich presents LC/MS/MS of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3, and 3-epi-25-Hydroxyvitamin D3 in Serum or Plasma on Ascentis® Express F5 after SPE with HybridSPE®-Phospholipid, Minimization of Matrix Effects
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Sample preparations, Solid phase extractions, Vitamins

Mitigation of Deuterium Scrambling in Stable-Labeled Internal Standards during LC-MS MS Analysis

Improvements in LC-MS/MS sensitivity and multiple reaction monitoring (MRM) capabilities are resulting in further adoption of LC-MS/MS technology in clinical settings. One specific clinical challenge...
Authors: Joshua Cooper1, Billy Molloy2, Huahua Jian1, Lisa Calton2, Derrell Johnson1, Isil Dilek1, Uma Sreenivasan1, Don Cooper2
1Cerilliant Corporation, 811 Paloma Drive, Suite A, Round Rock, TX 78665 2 Waters Corporation, Atlas Park, Simonsway, Manchester, M22 5 PP UKCONCLUSIONS
Keywords: Clinical, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Nuclear magnetic resonance spectroscopy, Phase transitions

Snap-N-Spike® reference solutions of 25-Hydroxyvitamin D

Over the last decade, vitamin D testing has increased significantly in the clinical laboratory setting as research studies have linked vitamin D deficiency to numerous diseases from diabetes, heart d...
Keywords: Clinical, Diabetes, Diseases, High performance liquid chromatography, Ion-exchange chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Vitamins

Vitamins | Technical Article

Sigma-Aldrich's Cerilliant brand provides certified Snap-N-Spike® | Snap-N-Shoot® solutions of numerous vitamins from the B, A, K and E groups to vitamin D including 25-Hydroxy and 1,25-Dihydroxy ana...
Keywords: Clinical, Degradations, Vitamins

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

H-073 25-Hydroxyvitamin D2 solution, 50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
H-074 D6-25-Hydroxyvitamin D3 (26,26,26,27,27,27-D6) solution, 50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
H-083 25-Hydroxyvitamin D3 solution, 100 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
H-087 25-Hydroxyvitamin D2 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
H-089 1α,25-Dihydroxyvitamin D3 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
H-090 1α,25-Dihydroxyvitamin D2 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
42579 4-Phenyl-1,2,4-triazoline-3,5-dione, for HPLC derivatization, LiChropur, ≥98.0% (CHN)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?