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H-089 Supelco

1α,25-Dihydroxyvitamin D3 solution

5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

  • CAS Number 32222-06-3

  • Empirical Formula (Hill Notation) C27H44O3

  • Molecular Weight 416.64

  •  EC Number 200-578-6

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Properties

Related Categories Additional Standards, Analytical Standards, Analytical/Chromatography, Biomarkers and Metabolomics Standards, Certified Reference Materials (CRMs),
grade   certified reference material
InChI Key   GMRQFYUYWCNGIN-KWJBEKLWSA-N
feature   Snap-N-Spike®/Snap-N-Shoot®
packaging   ampule of 1 mL
mfr. no.   Cerilliant®
concentration   5 μg/mL in ethanol
format   single component solution
storage temp.   −70°C

Description

General description

A Certified Spiking Solution® suitable for critical quantitative applications in research and clinical environments including: for use in preparation of controls and calibrators for LC/UV and LC-MS/MS Vitamin D assays; as system tuning/suitability standards; linearity verification; internal proficiency testing; and for OEM use in preparation of reagents, development of assays and instrument IQ/OQ. The stable, quantitative solution format provides significant benefits to the user including material savings, increased efficiency of labor, and convenience of use. The shelf stable Snap-N-Spike® format allows laboratories to eliminate painstaking weighing operations and provides the ability to spike a known concentration of analyte into their matrix of choice just before use.

Legal Information

CERILLIANT is a registered trademark of Cerilliant Corporation

CERTIFIED SPIKING SOLUTION is a registered trademark of Cerilliant Corporation

Snap-N-Shoot is a registered trademark of Cerilliant Corporation

Snap-N-Spike is a registered trademark of Cerilliant Corporation

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN1170 - class 3 - PG 2 - Ethanol, solution
WGK Germany 
1
Flash Point(F) 
57.2 °F
Flash Point(C) 
14 °C

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Hormone Standards

Over the past 17 years, hormone testing has increased significantly in the clinical laboratory setting as advances in the specificity and selectivity of LC-MS/MS technology have enabled hormones to s...
Keywords: Clinical, Clinical Chemistry, Diagnostic, Diseases, Endocrinology, High performance liquid chromatography, Hormones, Ion-exchange chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites

Improvement in LC/MS Analysis of Vitamin D Metabolites in Serum by Leveraging Column Selectivity and Effective Sample Prep

Clinical interest in vitamin D stems from its implications in an ever-widening range of human health conditions.1 This heightened interest has spawned the need for analytical strategies to assess an ...
Craig Aurand
Reporter US Vol 31.1
Keywords: Clinical, High performance liquid chromatography, Immunoassay, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Metabolites, Precipitation, Sample preparations, Separation, Solid phase extractions, Solvents, Vitamins

LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum

The unique combination of column selectivity using Ascentis Express F5 and phospholipid removal using HybridSPE-PLus provides a robust and accurate LC-MS method for accurate and sensitive analysis of...
Craig Aurand, Senior Application Chemist and Hugh Cramer, Associate Scientist
Reporter 32.1
Keywords: Buffers, Enzyme-linked immunosorbent assay, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Phase transitions, Precipitation, Reductions, Sample preparations, Separation, Solvents, Vitamins

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 (Monitored Ions)

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 (Monitored Ions)
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Solvents, Vitamins

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Solvents, Vitamins

Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum

The unique combination of column selectivity using Ascentis Express F5 and phospholipid removal using HybridSPE-PLus provides a robust and accurate LC-MS method for accurate and sensitive analysis of...
Craig Aurand, Hugh Cramer
Reporter US Volume 32.1
Keywords: Buffers, Enzyme-linked immunosorbent assay, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Phase transitions, Precipitation, Reductions, Sample preparations, Separation, Solvents, Vitamins

Snap-N-Spike® reference solutions of 25-Hydroxyvitamin D

Over the last decade, vitamin D testing has increased significantly in the clinical laboratory setting as research studies have linked vitamin D deficiency to numerous diseases from diabetes, heart d...
Keywords: Clinical, Diabetes, Diseases, High performance liquid chromatography, Ion-exchange chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Vitamins

Vitamins | Technical Article

Sigma-Aldrich's Cerilliant brand provides certified Snap-N-Spike® | Snap-N-Shoot® solutions of numerous vitamins from the B, A, K and E groups to vitamin D including 25-Hydroxy and 1,25-Dihydroxy ana...
Keywords: Clinical, Degradations, Vitamins

Peer-Reviewed Papers
15

References

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