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03-232 Sigma-Aldrich

Ubiquitin activating enzyme E1 (His-tag) human recombinant

Human recombinant Ubiquitin activating enzyme E1 (His-tag).

  •  eCl@ss 32160405

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   100
biological source   Escherichia coli
  human
mol wt   Mw ~119 kDa
mfr. no.   Upstate®
application(s)   activity assay: suitable
UniProt accession no.   P22314
shipped in   dry ice

Description

General description

Human recombinant Ubiquitin activating enzyme E1 (His-tag)

Product Source: Full length human ubiquitin-activating enzyme E1 containing a C-terminal His-tag

Ubiquitin activating enzyme E1 is responsible for the first step in the formation of the ubiquitin-protein isopeptide bond. It is a critical component for the initiation of in vitro conjugation reactions. E1 activates ubiquitin by first adenylating, with ATP, the C-terminal glycine carboxyl group of ubiquitin and thenlinking this residue to the sulphydryl side chain moiety of a cysteine residue in E1, by forming a high energy thiol ester bond and liberating free AMP. There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade.

Application

Human recombinant Ubiquitin activating enzyme E1 (His-tag).

Research Category
Cell Signaling

Epigenetics & Nuclear Function

Physical form

Format: Purified

In liquid containing 50 mM Tris-HCl, pH 7.5, containing 1.0 mM DTT. Frozen solution.

Storage and Stability

Stable for 12 months at -80°C from date of receipt. Avoid multiple freeze/thaw cycles.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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