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04-001 Sigma-Aldrich

Anti-JAK2 Antibody, clone 8E10.2

clone 8E10.2, Upstate®, from mouse

Synonym: Janus kinase 2, Tyrosine kinase

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, J, Primary Antibodies
clone   8E10.2, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   rat, mouse, human
shipped in   ambient
packaging   antibody small pack of 25 μg
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NP_004963
UniProt accession no.   O60674

Description

General description

JAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerizes and translocates to the nucleus and bind to CIS elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.

Specificity

This antibody recognizes Jak2.

Immunogen

KLH-conjugated linear peptide.

Application

Western Blot Analysis: 0.5-1 μg/mL of this lot was used to detect Jak2 in L6 cell lysates.

Immunohistochemistry(Paraffin):
A previous lot of this antibody was used in immunohistochemistry(paraffin) staining with eptiope retrieval: Human Cancer Array.

Anti-JAK2 Antibody, clone 8E10.2 detects level of JAK2 & has been published & validated for use in WB, IH(P).

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

Target description

130 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality

Routinely evaluated by western blot on L6 cell lysate.

Analysis Note

Control
L6 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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