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  • 04-035 - Anti-PTEN Antibody, clone 6H2.1

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04-035 Sigma-Aldrich

Anti-PTEN Antibody, clone 6H2.1

clone 6H2.1, Upstate®, from mouse

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, PT-PY, Primary Antibodies
clone   6H2.1, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable (paraffin)
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse, human, rat
shipped in   dry ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NP_000305.3
UniProt accession no.   O08586
  P60484
Gene Information   human ... PTEN(5728), TEP1(7011)

Description

General description

The protein and lipid phosphatase PTEN (phosphatase and tensin homolog deleted on chromosome ten) has been suggested to play a role in tumorgenesis. PTEN is a tumor suppressor whose loss permits constitutive signaling through the PI3K pathway and is sufficient to cause cancer. In normal cells, in conjunction with FOXO1A and PTEN function together to restrain cell growth. PTEN metabolizes the product of PI3K phosphorylation of PI(4,5)P2 following receptor activation, creating PI(3,4,5)P3. In cells lacking PTEN, there are elevated levels of PI(3,4,5)P3, which acts as a potent second messenger promoting oncogenesis. Restoration of PTEN function is considered a novel approach to cancer therapy. Overexpression of PTEN inhibits cell migration, and expression of antisense PTEN mRNA enhances migration.

Specificity

recognizes PTEN

Immunogen

C-terminal 100 amino acids of PTEN

Epitope: C-terminal region

Application

Anti-PTEN Antibody, clone 6H2.1 detects level of PTEN & has been published & validated for use in WB, IP, IH(P).

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Target description

55 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse IgG in storage buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality

routinely evaluated on HeLa cell lysate

Linkage

Replaces: 07-016

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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